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Assessment of drought response in local Scots pine ecotypes using anatomical, biochemical, and fluorescence stress markers.
Štěpánová, Kristýna ; Lhotáková, Zuzana (advisor) ; Stejskal, Jan (referee)
Global climate change and associated global warming is already a problem for temperate forest ecosystems and will continue to exacerbate the problem in the coming decades. Many species will have to adapt to a multifactorial combination of stresses, with increases in average air temperature, frequency and intensity of precipitation increasing drought episodes and drought-affected areas will enlarge. It is necessary to find ways to help the landscape cope with these intensifying phenomena. The possibility of planting suitable tree species that can manage water efficiently and survive even in periods of intense water scarcity is an option. The drought tolerance of trees is related to their phenotypic plasticity and ecotypic differentiation. Scots pine (Pinus sylvestris L.) is characterised by modest ecological demands on the environment and climate of the habitat, is phenotypically plastic and relatively drought resistant. The objectives of this study were 1) to evaluate and compare the response of different Scots pine ecotypes to drought stress due to reduced water availability using anatomical, biochemical, and fluorescence indicators of physiological status, and 2) to determine the biochemical phenotype of selected genotypes within a local Scots pine ecotype in a seed orchard in terms of...
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The effect of elevated CO2 concentration and irradiation on quantitative parameters of mesophyll cells of Norway spruce
Kubínová, Zuzana ; Albrechtová, Jana (advisor) ; Radochová, Barbora (referee)
KUBÍNOVÁ, Zuzana. The effect of elevated CO2 concentration and irradiation on quantitative parameters of mesophyll cells of Norway spruce. Prague, 2010. 74 p. Master's degree thesis. Faculty of Science, Charles University in Prague. Abstract The aim of the present thesis was to choose and adjust a suitable methodology for counting particles in 3D space, which would be suitable for unbiased estimation of chloroplast number in needle mesophyll cells. The disector method was used for the first time to determine the number of chloroplasts. This method enables unbiased estimation of chloroplast number in needle volume from optical sections captured from fresh free-hand sections by confocal microscope. The sections did not need any pre-processing. Another aim was to compare selected photosynthetic and anatomical characteristics of sun and shade Norway spruce needles, which were grown under different CO2 concentration. The trees were grown for eight years in ambient (during the experiment increasing from 357 up to 370 µmol CO2 ∙ mol-1 ) CO2 concentration or elevated (700 µmol ∙ mol-1 ) CO2 concentration in special glass domes on an experimental research site of the Institute of Systems Biology and Ecology, Academy of Sciences of the Czech Republic at Bílý Kříž in Moravskoslezské Beskydy mountains. The sun needles...
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