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Metabolism of new polysacharidic nanomaterials for biomedicinal applications
Jirátová, Markéta ; Hrubý, Martin (advisor) ; Jirák, Daniel (referee)
Cancer is one of the leading cause of death in modern world, so there is an emerging demand for better diagnostic tools and more specific less toxique therapeutics. Nanoparticles offers characteristics that could fullfill such perspectives. They can easily target tumor by ehanced permeation and retention effect (EPR). Nanoparticles can combine more than one imaging properties, so we can say that they are multimodal, some of them could combine diagnostic and therapeutic molecules in one nanoparticle, which is now highly popular topic of nanoparticles for theranostics . The aim of this thesis was to characterize new multimodal glycogen-based nanoparticle. Glycogen is an ideal structure for nanoparticle design. Glycogen is part of natural dendrimers group which are easily to modify. Glycogen's size is suitable for EPR effect. We have evaluated biological characteristics of five different types of modified glycogen. The in vitro experiments were carried on HepG2 cells. We have set time curve of cellular uptake of this glycogen probes, evaluated cytoplasmatic localization and for the first time we have carried MTT assay. Biodistribution studies on CD1-Nude mice were performed by using non-invasive method for measuring in vivo fluorescence. In conlusion we've provided some of the biological characteristics of new...
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Metabolism of new polysacharidic nanomaterials for biomedicinal applications
Jirátová, Markéta ; Hrubý, Martin (advisor) ; Jirák, Daniel (referee)
Cancer is one of the leading cause of death in modern world, so there is an emerging demand for better diagnostic tools and more specific less toxique therapeutics. Nanoparticles offers characteristics that could fullfill such perspectives. They can easily target tumor by ehanced permeation and retention effect (EPR). Nanoparticles can combine more than one imaging properties, so we can say that they are multimodal, some of them could combine diagnostic and therapeutic molecules in one nanoparticle, which is now highly popular topic of nanoparticles for theranostics . The aim of this thesis was to characterize new multimodal glycogen-based nanoparticle. Glycogen is an ideal structure for nanoparticle design. Glycogen is part of natural dendrimers group which are easily to modify. Glycogen's size is suitable for EPR effect. We have evaluated biological characteristics of five different types of modified glycogen. The in vitro experiments were carried on HepG2 cells. We have set time curve of cellular uptake of this glycogen probes, evaluated cytoplasmatic localization and for the first time we have carried MTT assay. Biodistribution studies on CD1-Nude mice were performed by using non-invasive method for measuring in vivo fluorescence. In conlusion we've provided some of the biological characteristics of new...
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Dědičné nádorové systémy u člověka
ŘÍHOVÁ, Lenka
The subject of this thesis is an overview of cancer syndromes in humans. The thesis focuses on some of the major genes that cause a predisposition to cancer. These genes were studied: BRCA1, BRCA2, BLM, ATM, PAL2, RAD51 paralogs, CHEK2, APC, TP53, MSH2, MLH1, Rb1, CDK4, CDKN2A. There are also presented some major representatives of hereditary cancer syndromes with a focus on the symptoms and long-term monitoring of the disease. These diseases were described: Ataxia-telangiectasia, Bloom syndrome, Li-Fraumeni syndrome, familial adenomatous polyposis and Hereditary breast and ovarian cancer. The thesis also deals with methods of detection of mutations within genetic diagnosis, in which especially New generation sequencing plays an important role.
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