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National Repository of Grey Literature

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Lichens of Trubínský vrch hill near Beroun (central Bohemia) and a study of Aspicilia dominiana Lenzová, Veronika ; Svoboda, David (advisor) ; Kocourková, Jana (referee) Lichens of Trubínský vrch hill near Beroun (central Bohemia) and a study of Aspicilia dominiana Veronika Lenzová Abstract This diploma thesis is divided into two separate parts. The goal of the first part was to carry out a complete lichenofloristic research of Trubín hill near Beroun. There are diabasic outcrops on the south side of the hill. Due to wide spectrum of the lichen species this substrate is very interesting for the lichenologists. In total I have found 125 lichen species there. 105 lichen species grow on diabasic rocks. A considerable amount of recorded lichens is among endangered species for example Aspicilia dominiana, Caloplaca conversa, Lichinella stipatula, Phaeophyscia constipata. The second part is focused on the taxonomic study of the Aspicilia dominiana and related species. A. domininana is recently known only from a few diabasic localities in the Czech Republic. After revision of the herbarium material based on microscopic and chemical characteristics I have found out that the taxon A. dominiana is similar with the A. intermutans. Compliance of these taxa have been confirmed with the molecular data from the sequencing of the two genes nrITS and mTSS too. Therefore I propose to synonymize these two taxa. According to the phylogenetic tree the lichen A. intermutans is not closely... Detailed record

A taxonomic study of Physconia muscigena group Starosta, Jakub ; Svoboda, David (advisor) ; Šoun, Jaroslav (referee) The principal goal of my study was to find if ecologically and chemically different populations of lichens in the Physconia muscigena (Ach.) Poelt group belong to several species or a single one. This study focused on the molecular and chemical investigations of mostly European and Canadian populations. I use sequence data from three genes (ITS rDNA, mtSSU rDNA and TEF1) for the reconstruction of phylogenetic trees. I investigate phylogenetic relationships among the closely related species P. muscigena, P. bayeri, P. rossica, and P. isidiomuscigena. Also, I wanted to detect any possible geographical or ecological trends among chemotypes and haplotypes. As an additional goal I checked the recent localities of P. muscigena in the Czech Republic for valorising its conservation status. Results show that: (1) sequenced data of ITS rDNA and TEF1 show high intraspecific variability in P. muscigena samples. This genetic variability does not correlate neither with geographical distribution nor thallus chemistry; (2) P. bayeri is synonymous with P. muscigena; (3) some samples P. muscigena contain new undetermined secondary metabolite, (4) Physconia muscigena has only three recent localities in Czech Republic. Key words: Physconia muscigena, Physconia bayeri, infraspecific variability, taxonomy, TEF1, ITS... Detailed record

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