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Molecular cytogenetic analysis of marker chromosomes and related abnormalities
Semanko, Adam ; Kočárek, Eduard (advisor) ; Holá, Dana (referee)
The primary focus of this diploma thesis is on marker chromosomes and phenotypically similar human karyotype polymorphisms, variants of short acrocentric arms in particular. The first half provides a very useful review of literature concerning different aspects of both sSMC and human polymorphisms such as their origin, inheritance, associated phenotype, formation and molecular cytogenetic methods that are applied in the process of identification of these aberrations. The methodical emphasis is on FISH and its modifications (e.g. M-FISH, acro M FISH, cen M-FISH) as well as on the CGH methods. The main objective was to test the above-mentioned methods and state their limitations and applications. Thus, in the other half we provide evaluations of commonly used methods and introduce new strategies that could be implemented to make the identification of these additional chromosomes or satellite translocations more effective. All the conclusions are based on the analysis of 7 patients with sSMC and 4 patients with variants involving acrocentric NOR regions. The results of our thorough research into their karyotypes have been compared with similar findings in the literature. Last but not least, we tried to establish a link between observed abnormalities and the type of a chromosomal aberration at hand.
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Mythological deities and beings associated with water depicted in the mosaics of ancient Rome
Odler, Ján ; Ondřejová, Iva (advisor) ; Titz, Pavel (referee)
This thesis focuses on the mosaic scenes of Roman era, especially on the gods and other mythological figures associated with water. The thesis is divided into 10 chapters. The first chapter is dedicated to the mythology of gods and characters and their brief iconographic characterization. Other chapters are divided by the areas that were under domination of Roman Empire. Each of these chapters has identical structure. The text deals with the mosaics and motifs of these areas in general. Then there are described subsequently collected examples of mosaics. On the basis of the description and characteristics is the last chapter sub-section dealing with similarities and comparison. The penultimate chapter focuses on a mosaic in terms of several aspects: production, technology, workshops, location, different techniques, signatures and trademarks. The conclusions that result from surveyed sample mosaics are summarized at the end of thesis. It is especially focused on the motifs and characters. Keywords Iconography, mosaics, Roman art, deities
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Mythological deities and beings associated with water depicted in the mosaics of ancient Rome
Odler, Ján ; Ondřejová, Iva (advisor) ; Titz, Pavel (referee)
This thesis focuses on the mosaic scenes of Roman era, especially on the gods and other mythological figures associated with water. The thesis is divided into 10 chapters. The first chapter is dedicated to the mythology of gods and characters and their brief iconographic characterization. Other chapters are divided by the areas that were under domination of Roman Empire. Each of these chapters has identical structure. The text deals with the mosaics and motifs of these areas in general. Then there are described subsequently collected examples of mosaics. On the basis of the description and characteristics is the last chapter sub-section dealing with similarities and comparison. The penultimate chapter focuses on a mosaic in terms of several aspects: production, technology, workshops, location, different techniques, signatures and trademarks. The conclusions that result from surveyed sample mosaics are summarized at the end of thesis. It is especially focused on the motifs and characters. Keywords Iconography, mosaics, Roman art, deities
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Development of Greek mosaics in the Hellenistic period
Pejchlová, Tereza ; Stančo, Ladislav (advisor) ; Ondřejová, Iva (referee)
This Master thesis deals with the development, mainly the technological development of the Greek mosaics in the Hellenistic period. Mosaics of the late Classical period are also included in this work as the early Hellenistic mosaic pavements continued to use and improve the late Classical technique. With respect to the technological aspect it is important to describe the production process, sources of which also exist in the ancient literature. The working environment and the organization of the mosaicists or other craftsmen involved can be to some extent reconstructed based on the archaeological finds. The next part of the thesis is concerned with the architectural context, the function and the meaning of the Greek mosaics. Certain hierarchy of the mosaic importance and the meaning can be identified on the basis of their motifs, the technique of the performance and their location inside the building. Furthermore, it is examined, how spaces of private houses changed with mosaics from the late Classical to the late Hellenistic times. In closing there is basic information about the Hellenistic mosaic repertory presented. Keywords mosaics, Greek, Hellenism, Art, Hellenistic, development
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Molecular cytogenetic analysis of marker chromosomes and related abnormalities
Semanko, Adam ; Kočárek, Eduard (advisor) ; Holá, Dana (referee)
The primary focus of this diploma thesis is on marker chromosomes and phenotypically similar human karyotype polymorphisms, variants of short acrocentric arms in particular. The first half provides a very useful review of literature concerning different aspects of both sSMC and human polymorphisms such as their origin, inheritance, associated phenotype, formation and molecular cytogenetic methods that are applied in the process of identification of these aberrations. The methodical emphasis is on FISH and its modifications (e.g. M-FISH, acro M FISH, cen M-FISH) as well as on the CGH methods. The main objective was to test the above-mentioned methods and state their limitations and applications. Thus, in the other half we provide evaluations of commonly used methods and introduce new strategies that could be implemented to make the identification of these additional chromosomes or satellite translocations more effective. All the conclusions are based on the analysis of 7 patients with sSMC and 4 patients with variants involving acrocentric NOR regions. The results of our thorough research into their karyotypes have been compared with similar findings in the literature. Last but not least, we tried to establish a link between observed abnormalities and the type of a chromosomal aberration at hand.
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Methodology of monitoring and maintenance of exterior glass mosaics
Kučerová Irena ; Zlámalová Cílová, Zuzana ; Novák, Michal ; Holubová, Barbora ; Knotek, Vítězslav ; Knězů Knížová, Michaela
Metodika se v úvodu věnuje problematice poškození exteriérových skleněných mozaik s ohledem na korozi a degradaci malty a mozaikového skla. Hlavní část metodiky je zaměřena na monitoring, dokumentaci monitoringu a pravidelnou údržbu skleněných exteriérových mozaik. Podrobně řeší problematiku čištění mozaikového skla a upozorňuje na možná negativa jednotlivých metod. V další části popisuje přípravu ochranných lakových vrstev pro ochranu mozaikového skla a uvádí přípravu laku, který může nahradit organokřemičitan HS-56TF/A (výrobce Tokuyama), který byl původně použitý při restaurování mozaiky Posledního soudu v letech 1998-2000. Tento přípravek je třeba nahradit, protože se původně užitý přípravek nevyrábí. V závěru se metodika zabývá využitím pružných tmelů pro tmelení prasklin a shrnuje pozitiva a negativa jejich aplikace při údržbě exteriérových skleněných mozaik.
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