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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Compartmentalisation of surface receptor signalling in T cells
 Paldusová, Kateřina ; Cebecauer, Marek (advisor) ; Janušová, Šárka (referee)
The main goal of this thesis is to introduce the topic of compartmentalisation of signalling on the surface of T lymphocytes before and during the T-lymphocyte activation by the contact with an antigen-presenting cell (APC) or a target cell. To understand the compartmentalisation of signalling, the morphology of cell surface needs to be understood first. It is believed that the surface of T cell is magnified with an abundance of membrane protrusions called microvilli. Although, little is known about their inner structure. They may have some structural features different from microvilli on the surface of APC or microvilli of intestinal brush border. These structures are further described and compared with each other along-side with some other non-microvillar membrane protrusions and extensions. The insight into three-dimensional compartmentalisation of signalling molecules in T cells is still in its early days. Some results even contradict each other. This field will require the application of advanced imaging methods in a rather comprehensive way to uncover fine organisation of these molecules before and after encountering stimulatory surface. Key words T-cell signalling, membrane receptors, cell surface morphology, microvilli, microscopy
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Quantitative fluorescence microscopy techniques to study three-dimensional organisation of T-cell signalling molecules.
 Chum, Tomáš ; Cebecauer, Marek (advisor) ; Lánský, Zdeněk (referee) ; Brameshuber, Mario (referee)
10 SUMMARY Proteins represent one of the basic building blocks of all organisms. To understand their function at the molecular level is one the critical goals of current biological, biochemical and biophysical research. It is important to characterise all aspects that affect the localisation of proteins into different compartments with specific functions, the dynamic structure of proteins and their role in multiprotein assemblies, because altering these properties can lead to various diseases. Most of the proteomic studies are nowadays performed using biochemical approaches that allow us to study multicellular organism or tissue at once. The disadvantage of these methods is complex preparation of sample and the need for a large number of cells, which leads to the loss of information at the molecular level and in individual cells. On the contrary, microscopy can provide rather detailed information about proteins of interest and at the level of a single cell. A variety of fluorescence microscopy methods in combination with recombinant DNA techniques were applied to elucidate subcellular localisation of transmembrane adaptor proteins (TRAPs) in human lymphocytes and their nanoscopic organisation at the plasma membrane. Linker of activation of T lymphocytes (LAT), phosphoprotein associated with...
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