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Advanced Computational Methods for Increasing the Discriminatory Power of Genotyping Methods Nykrýnová, Markéta ; Budinská, Eva (referee) ; Hrabák,, Jaroslav (referee) ; Škutková, Helena (advisor) Tato disertační práce je zaměřena na vytvoření nových výpočetních metod, které zvýší diskriminačních schopnost genotypizačních metod. Hlavní důraz je kladen na odlišení blízce příbuzných bakterií, které pocházejí například z jedné nemocnice či jednoho oddělení. V první části práce jsou popsány současné typizační metody a jsou představeny nové postupy pro identifikaci genetických markerů s vysokou mírou sekvenční variability, pomocí kterých lze lépe rozlišit bakteriální populaci. Navržené metody jsou založeny na výpočtu signálů entropie a analýze nenamapovaných čtení. Druhá část práce se zabývá návrhem nových metod zpracování surových dat z nanopórového sekvenování, které lze použít pro rychlou vysoce citlivou typizaci bakterií bez nutnosti převádět proudové signály na nukleotidové sekvence. Předložená práce přispívá ke zlepšení a zpřesnění rutinně používaných typizačních metod pomocí navržených bioinformatických postupů a představuje unikátní přístup využití doposud experimentální techniky nanopórového sekvenování pro rychlou genotypizaci a analýzu bakterií. Detailed record

Genotyping Of Klebsiella Pneumoniae Isolates Nykrýnová, Markéta Typing methods capable of distinguishing bacterial strain are essential for epidemiologist because they help to prevent and control infections. Unfortunately, many typing methods are timeconsuming, expensive and reproducibility of their results is questionable. Therefore, we propose a new genotyping method, which is capable of identifying genes with high rate of variability to distinguish different bacterial strains. Detailed record

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