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Vliv polymorfismu kandidátního lokusu na technologické vlastnosti mléka
MIJAILOVIĆ, Angelina
The aim of this thesis is to analyze the influence of polymorphic variants of selected locus (SCD) on the technological quality of milk. At the beginning is described, milk yields along with the mammary gland - structure, diseases associated with it, colostrum and milk production. The composition of milk includes fat, fatty acids, carbohydrates, minerals, vitamins, proteins - whey protein (-lactoglobulin, -lactalbumin), casein (-casein, -casein, -casein) The thesis also deals with the genome of cattle, genetic markers, candidate loci. Methodology describes DNA isolation, PCR, including PCR for SCD, RFLP, and determination of fatty acids by spectrophotometry. At the end of the thesis are the results, which evaluate the genotypes and alleles abundance, the milk performance indicators depending on the genotype for SCD and the evaluation of the influence of the SCD genotype on the fatty acid spectrum.
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