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Analýza metylace DNA pomocí kapilární elektroforézy
 Zahálka, Martin
This bachelor thesis, DNA methylation analysis by capillary electrophoresis, deals with the analysis of methylated DNA by capillary electrophoresis. The theoretical part introduces the general principles of methylation, asymmetric and symmetric methylation, de novo methylation and final DNA methylation. The following chapter is devoted to the methylation of DNA in bacteria, plants and animals. The possibilities of detection of methylated DNA and their characterization are also elaborated in the literature review. At the end of the theoretical part is also devoted to the chapter capillary electrophoresis. The essence of the experimental part is the separation of cytosine and methylcytosine. Capillary electrophoresis was used to analyze standard cytosine and methylcytosine solutions. During the experiment, the separation electrolyte and the separation conditions were adjusted. Ideal conditions were achieved using a 50 mM borate buffer with the addition of sodium dodecyl sulfate (SDS) with a final concentration of 60 mM, at 20 kV and 30 mBar.
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