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Evaluation of grape pomace as carbon source for bacterial cellulose production
Stříž, Radim ; Obruča, Stanislav (referee) ; Kovalčík, Adriána (advisor)
This bachelor thesis is focused on the production of bacterial cellulose by Gluconacetobacter xylinus. The theoretical part consists of a brief description of G. xylinus, properties and uses of bacterial cellulose. Firstly, the experimental part focuses on optimization of production of bacterial cellulose by G. xylinus CCM 4611. This optimization was focused on pH value, a cultivation type, and used carbon source for production. The optimal pH was 6.5. The carbon source showing the highest production of bacterial cellulose was mannose for the dynamic production and saccharose for the static production of bacterial cellulose. The second part of the experimental part focuses on a comparison of two methods for assaying reducing sugars – Somogyi-Nelson (SN) assay and dinitrosalicylic acid (DNS) assay. The comparison of two above mentioned methods showed that the results obtained by the SN method were very close to High performance liquid chromatography (HPLC) data. It can be concluded that HPLC method is the best method if we need to know the type of sugars but if we need only the quick determination of the sum of the reduction sugars, SN method is very suitable. 4 It can be concluded that the Moravian region produces a large amount of winery biomass. Therefore, the use of the pomace extract as a carbon source for the production of bacterial cellulose seems to be interesting mainly from the point of the recirculation economy. The obtained results showed that the grape sugar extract could be used, and from the production values, it is even more suitable for the production of bacterial cellulose than industrial sugars.
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Evaluation of grape pomace as carbon source for bacterial cellulose production
Stříž, Radim ; Obruča, Stanislav (referee) ; Kovalčík, Adriána (advisor)
This bachelor thesis is focused on the production of bacterial cellulose by Gluconacetobacter xylinus. The theoretical part consists of a brief description of G. xylinus, properties and uses of bacterial cellulose. Firstly, the experimental part focuses on optimization of production of bacterial cellulose by G. xylinus CCM 4611. This optimization was focused on pH value, a cultivation type, and used carbon source for production. The optimal pH was 6.5. The carbon source showing the highest production of bacterial cellulose was mannose for the dynamic production and saccharose for the static production of bacterial cellulose. The second part of the experimental part focuses on a comparison of two methods for assaying reducing sugars – Somogyi-Nelson (SN) assay and dinitrosalicylic acid (DNS) assay. The comparison of two above mentioned methods showed that the results obtained by the SN method were very close to High performance liquid chromatography (HPLC) data. It can be concluded that HPLC method is the best method if we need to know the type of sugars but if we need only the quick determination of the sum of the reduction sugars, SN method is very suitable. 4 It can be concluded that the Moravian region produces a large amount of winery biomass. Therefore, the use of the pomace extract as a carbon source for the production of bacterial cellulose seems to be interesting mainly from the point of the recirculation economy. The obtained results showed that the grape sugar extract could be used, and from the production values, it is even more suitable for the production of bacterial cellulose than industrial sugars.
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