National Repository of Grey Literature 2 records found  Search took 0.02 seconds. 
Touha po cukru; fyziologické procesy klíštěte \kur{Ixodes ricinus} v závislosti na hostitelské glukóze
PLAČKOVÁ, Barbora
Blood-feeding arthropods, Ixodes ricinus acquire and digest vast amounts of the host blood meal, which often exceeds their body volume up to 100 times. It seems to be an only source of energy which they need for the reproduction. I identified homologues of six sugar transporters genes encoding sugar transporters in the I. ricinus tick. Transcripts encoding sugar transporters were found in digestive tract of I. ricinus. The results of RT PCR analysis characterised tissue expression profiles of the sugar transporters while fed by blood. The sugar transporters were determined as a drug target by RNAi. In this master thesis, the use of RNAi and available sugar inhibitors did not confirm glucose importance in physiology of ticks. Membrane feeding showed that blood meal with 2-deoxy-D-glucose had a significant impact on the reproduction and viability of the ticks.
\kur{Ixodes holocyclus\kur{}} - životní cyklus, toxicita a schopnost přenášet borrelie
VLČKOVÁ, Kamila
Ixodes holocyclus is the most common species of blood-feeding arthropods along the whole eastern coast of Australia. The salivary secretions contain neurotoxins, which cause paralysis and toxicoses to the hosts, especially cats, dogs, livestock, and even man, while native fauna appears to be immune. Despite its wide distribution, the role of paralytic toxins in disease transmission and the spectrum of pathogens carried by this tick are poorly understood. It is impossible to breed the ticks on animals because of their toxicity. However using an artificial feeding membrane helped us to establish a laboratory colony, which was necessary to perform our subsequent experiments. This study was focused on the ability of I. holocyclus to transmit Borrelia afzelii, a spirochete bacterium causing Lyme borreliosis. The next goal was to identify the paralytic neurotoxins.

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