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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Possibilities of affecting secondary metabolites production in "in vitro" cultures of medicinal plants
 Sojková, Kristýna ; Tůmová, Lenka (advisor) ; Martin, Jan (referee)
1 ABSTRACT Possibilities of affecting secondary metabolites production in in vitro cultures of medicinal plants Elicitation is one of the methods used for increasing the production of secondary metabolites.The aim of this work was to affect the flavonoid production in callus and suspension cultures of St. John's Wort (Hypericum perforatum L.). Abiotic elicitor selendioxide in various concentrations (9.012.10-3 mol/l; 9.012.10-4 mol/l; 9.012.10-5 mol/l) was added in in vitro culture. The content of flavonoids was determinated by high performance liquid chromatography (HPLC). The samples were taken after 6, 12, 24, 48, 72, 168 hours after elicitor treatment. Selendioxide raised the production of hyperoside and quercitrin. The highest content of hyperoside (0.2 %) was detected after selendioxide (in concentration of 9.012.10-3 mol/l) treatment and 168 hours sampling in suspension culture. The highest production of quercitrin (0.37 %) was reached after selendioxide treatment (in concentration of 9.012.10-3 mol/l) and 6 hours sampling also in suspension culture. Production of flavonoids in callus cultures wasn't so much affected in comparison with suspension cultures after elicitor treatment. Flavonoid releasing into nutrient medium was also studied. The highest content of hyperosid (1.02 mg/100ml) was found in...
Detailed record
Possibilities of affecting secondary metabolites production in "in vitro" cultures of medicinal plants
 Sojková, Kristýna ; Tůmová, Lenka (advisor) ; Martin, Jan (referee)
1 ABSTRACT Possibilities of affecting secondary metabolites production in in vitro cultures of medicinal plants Elicitation is one of the methods used for increasing the production of secondary metabolites.The aim of this work was to affect the flavonoid production in callus and suspension cultures of St. John's Wort (Hypericum perforatum L.). Abiotic elicitor selendioxide in various concentrations (9.012.10-3 mol/l; 9.012.10-4 mol/l; 9.012.10-5 mol/l) was added in in vitro culture. The content of flavonoids was determinated by high performance liquid chromatography (HPLC). The samples were taken after 6, 12, 24, 48, 72, 168 hours after elicitor treatment. Selendioxide raised the production of hyperoside and quercitrin. The highest content of hyperoside (0.2 %) was detected after selendioxide (in concentration of 9.012.10-3 mol/l) treatment and 168 hours sampling in suspension culture. The highest production of quercitrin (0.37 %) was reached after selendioxide treatment (in concentration of 9.012.10-3 mol/l) and 6 hours sampling also in suspension culture. Production of flavonoids in callus cultures wasn't so much affected in comparison with suspension cultures after elicitor treatment. Flavonoid releasing into nutrient medium was also studied. The highest content of hyperosid (1.02 mg/100ml) was found in...
Detailed record

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