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Factors influencing the quality of red wine
Zechmeisterová, Lucie ; Vránová, Dana (referee) ; Omelková, Jiřina (advisor)
In my thesis, I focused on monitoring of microorganisms in the sample of red grape juice and on the interactions between yeasts, bacteria and filamentous fungi. Three different media were applied for the cultivation of microorganisms; firstly for monitoring of total volume of microorganisms, secondly for yeasts and third time for lactic acid bacteria. The indirect method was used for the determination of the amount of viable cells. This method consists in enumerating of visible macroscopic colonies grown up on agar plates. When the cells grew up, the forms of colonies were analyzed visually and the morphology of microorganisms was detected microscopically. The operating time of enzymes in grape juice in the production of red wine was monitored after application of commercial enzymatic preparation. The enzym action in grape juice was observed on the basis of the process of degradation of high – molecular substrate by enzymes through the use of Ubbelohd´s viscometer. The research findings provided a lot of knowledge about the occurance of microflora in the process of production of red wine. The commercial preparations added to grape juice played a significant role.
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Microbiology of wine
Valicová, Markéta ; Navrátilová, Jana (referee) ; Omelková, Jiřina (advisor)
The aim of this work was the investigation of the amount and the composition of microflora during the winemaking of red wine. The study was performed in 3 factories in Velké Pavlovice region, where each of them utilized its own technological approach. According to this, during the period of preliminary fermentation, 3 different technological procedures were used, which differed in application of commercial yeasts and enzymes, and sulphur dioxide. They differed also in the temperature during the period of alcoholic fermentation and the application of commercial bacteria during the period of malolactic fermentation. From the experimental results follows that the application of different technological approaches leads to the differences in the number of both yeast and bacteria viable cells and the total number of microorganism cells in the period of preliminary, alcoholic and malolactic fermentation.
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Factors influencing the quality of red wine
Zechmeisterová, Lucie ; Vránová, Dana (referee) ; Omelková, Jiřina (advisor)
In my thesis, I focused on monitoring of microorganisms in the sample of red grape juice and on the interactions between yeasts, bacteria and filamentous fungi. Three different media were applied for the cultivation of microorganisms; firstly for monitoring of total volume of microorganisms, secondly for yeasts and third time for lactic acid bacteria. The indirect method was used for the determination of the amount of viable cells. This method consists in enumerating of visible macroscopic colonies grown up on agar plates. When the cells grew up, the forms of colonies were analyzed visually and the morphology of microorganisms was detected microscopically. The operating time of enzymes in grape juice in the production of red wine was monitored after application of commercial enzymatic preparation. The enzym action in grape juice was observed on the basis of the process of degradation of high – molecular substrate by enzymes through the use of Ubbelohd´s viscometer. The research findings provided a lot of knowledge about the occurance of microflora in the process of production of red wine. The commercial preparations added to grape juice played a significant role.
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Microbiology of wine
Valicová, Markéta ; Navrátilová, Jana (referee) ; Omelková, Jiřina (advisor)
The aim of this work was the investigation of the amount and the composition of microflora during the winemaking of red wine. The study was performed in 3 factories in Velké Pavlovice region, where each of them utilized its own technological approach. According to this, during the period of preliminary fermentation, 3 different technological procedures were used, which differed in application of commercial yeasts and enzymes, and sulphur dioxide. They differed also in the temperature during the period of alcoholic fermentation and the application of commercial bacteria during the period of malolactic fermentation. From the experimental results follows that the application of different technological approaches leads to the differences in the number of both yeast and bacteria viable cells and the total number of microorganism cells in the period of preliminary, alcoholic and malolactic fermentation.
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