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National Repository of Grey Literature

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Separation and purification of nucleic acids with the use of magnetic microparticles Vlachová, Jana ; Rittich, Bohuslav (referee) ; Španová, Alena (advisor) Application of magnetic microparticles for nucleic acid isolation from real samples is a modern and fast separation procedure. This method is suitable for application in laboratory practice. The aim of the bachelor work was isolation of DNA from a dairy product (Activia sweet white) in the PCR-ready quality. DNA isolation was carried out by magnetic microparticles P(HEMA co GMA) in the presence of 2 M NaCl and 16% PEG 6000 or 8% PEG 6000. According to the declaration of the producer the presence of DNA from probiotic bacteria of the genera Lactobacillus and Bifidobacterium was confirmed by PCR. Detailed record

Separation and purification of nucleic acids with the use of magnetic microparticles Vlachová, Jana ; Rittich, Bohuslav (referee) ; Španová, Alena (advisor) Application of magnetic microparticles for nucleic acid isolation from real samples is a modern and fast separation procedure. This method is suitable for application in laboratory practice. The aim of the bachelor work was isolation of DNA from a dairy product (Activia sweet white) in the PCR-ready quality. DNA isolation was carried out by magnetic microparticles P(HEMA co GMA) in the presence of 2 M NaCl and 16% PEG 6000 or 8% PEG 6000. According to the declaration of the producer the presence of DNA from probiotic bacteria of the genera Lactobacillus and Bifidobacterium was confirmed by PCR. Detailed record

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