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Effect of morphine preconditioning and mKATP channel activity on survival of differentiated SH-SY5Y cell line after oxidative stress
Paluba, Michal ; Hejnová, Lucie (advisor) ; Kolář, David (referee)
Morphine as one of the most effective analgesics has been used in medicine for more than a century. However, morphine is also known for its neuroprotective and cardioprotective effect in ischemic disorders, where it induces increased cellular resistance to oxidative stress. One of the aims of our study was to analyze the effect of chronic morphine treatment on the viability of differentiated cells of the neuroblastoma cell line SH-SY5Y after induction of oxidative stress by tert butyl hydroperoxide (tBHP). However, the detailed mechanism of the protective effect of morphine is still unknown. Current research on this topic, and in particular on morphine-induced preconditioning, has focused much attention on mitochondrial ATP- sensitive potassium channels (mKATPs). There is evidence that morphine, through activation of opioid receptors, mimics the protective effect of ischemic preconditioning precisely through activation of mKATP channels. Modulation of mKATP channel activity is thought to be responsible for the functional state of the mitochondria by altering mitochondrial membrane potential and regulating reactive oxygen species (ROS) production. Thus, the next aim of our work was to reveal the role of mKATP channels in morphine preconditioning and the effect of their activity on cell survival. The...
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