|
|
Study of low temperuture microwave plasma on yeats
Petrová, Veronika ; Kozáková, Zdenka (referee) ; Krčma, František (advisor)
This bachelor thesis subject is the study of low temperature microwave plasma torch on yeasts. For this purpose, the yeast Candida Glabrata was selected. Three parameters, which have different effects on the size of the inhibition zones and their time stability, are monitored. The effect of sample concentration, exposure time, and applied power (9 W and 12 W) are investigated. A microwave torch discharge at atmospheric pressure is used. The working gas is argon and a constant flow rate of 5.0 Slm was maintained throughout the experiment. The visible end of the discharge is in direct contact with the sample surface during the experiment. In the experimental part, although the microwave torch discharge used was found to be unstable, its sterilizing effect on yeast was demonstrated.
|
|
|
Study of low temperature plasma direct application on yeasts Candida Glabrata and bacteries
Petrová, Veronika ; Kodedová,, Marie (referee) ; Krčma, František (advisor)
The subject of this thesis is to study the interactions of the low-temperature microwave plasma with selected microorganisms Candida glabrata, Staphylococcus epidermidis, and Escherichia coli and their combinations. The inhibitory effect of the plasma on these microorganisms is monitored on selected solid nutrient media and in suspension (nutrient medium and PBS). The inhibitory effect on the mixed cultures of these microorganisms is also studied on the solid nutrient media. The surface wave microwave discharge is used, with argon as a working gas. For the experiment on the solid nutrient media, a constant gas flow rate of 5,0 Slm and a power fluctuating between 8-10 W are maintained throughout the experiment. For the suspension experiment, a constant gas flow rate of 2,0 Slm and 13 W power are maintained throughout the experiment. The inhibitory effects of plasma on the solid nutrient media were observed on the individual microorganisms as well as on the mixed cultures. It was found that the microorganisms treated by plasma on the solid nutrient media were inhibited and uncultivable, while their cytoplasmic membrane remained intact. When the microorganisms were treated in suspension, individual cells were killed, but the efficiency was low. This was caused by the experimental setup, specifically due to the plasma only being formed in argon (no intermixing with air), as the capillary was submerged in the treated suspension.
|
|
|
Study of low temperuture microwave plasma on yeats
Petrová, Veronika ; Kozáková, Zdenka (referee) ; Krčma, František (advisor)
This bachelor thesis subject is the study of low temperature microwave plasma torch on yeasts. For this purpose, the yeast Candida Glabrata was selected. Three parameters, which have different effects on the size of the inhibition zones and their time stability, are monitored. The effect of sample concentration, exposure time, and applied power (9 W and 12 W) are investigated. A microwave torch discharge at atmospheric pressure is used. The working gas is argon and a constant flow rate of 5.0 Slm was maintained throughout the experiment. The visible end of the discharge is in direct contact with the sample surface during the experiment. In the experimental part, although the microwave torch discharge used was found to be unstable, its sterilizing effect on yeast was demonstrated.
|
guest ::
