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Potential of brassinosteroids for forest seed management with regard to species utilizable as Christmas trees
Jílek, Jan ; Kuneš, Ivan (advisor) ; Olga, Olga (referee)
The aim of this work is to assess the effect of brassinosteroids on germination of selected forest tree species with a potential to be used for the production of Christmas trees.The synthetically prepared analog 24-epibrassinolide (was tested on germination of seeds of Scots pine (Pinus sylvestris L.). Prior to launch of germination, the seeds were being soaked for 48 hours at three different concentrations of the tested brassinosteroid (BR-treatments) and in distilled water only (control), respectively. Seeds of each above-mentioned treatment were exposed to two alternative regimes: the stressed and unstressed one, respectively. In the stressed regime, seeds were exposed to a stress episode with temperatures up to 42 °C. In the unstressed regime the temperatures followed a pattern prescribed by Czech seed testing rules. Fully germinated seeds (the length of root radicle at least 4 times longer than the size of seed) were calculated. The root radicle length of each germinated seed was measured as well.The best results were recorded in the BR-treatment with the highest concentration of the tested brassinosteroid (0.4 mg.dm-3) in both germination regimes. As contrasted to the control, the high concentration treatment increased germination capacity in the unstressed and stressed regime by 16.8% and 31%, respectively.

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