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Identification of selected probiotic bacteria in food additives
Bubeníková, Lucia ; Španová, Alena (referee) ; Rittich, Bohuslav (advisor)
Bifidobacteria and lactobacilli are gram-positive bacteria which belong to the lactic acid bacteria group. They are ordinary constituents of gastrointestinal microflora, play a pivotal role in human nutrition and health, prevent pathogen colonization and maintain normal mucosal immunity. In present days they are used in production of functional food products and pharmaceutical additives. Polymerase chain reaction (PCR) is used to the detection and identification of bacteria which belong to the genus of Bifidobacterium and Lactobacillus. Specific primers for each genus are used in PCR reactions for in vitro amplification of definite part of DNA. In this work, total DNA was isolated from three additives (Pangamin Bifi plus, Biopron Junior and Probiodom) by the method of phenol extraction and amplified using genus-specific PCR primers giving amplicons of 523 bp (Bifidobacterium) and 250 bp (Lactobacillus). Occurrence of bacteria of both genera was proved in all of the three tested probiotic products.
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Microbiological diagnosis of Ureaplasma urealyticum and Mycoplasma hominis in women with urogenital infections
SLÍPKOVÁ, Gabriela
This bachelor's thesis is focused on the diagnosis of bacteria Ureaplasma urealyticum and Mycoplasma hominis in women with urogenital infections. The theoretical part of the work deals with urogenital infections, such as urinary tract infections, reproductive tract infections system of women and their most common bacterial agents. The issue is described in the next section Ureaplasma urealyticum and Mycoplasma hominis. The methodology and goals of the thesis included laboratory evidence of mycoplasmas, as well as evidence of sensitivity to antibiotics by using some kits. Another task was laboratory processing of swabs from the vagina. Furthermore, the statistical evaluation of the results of patients in Unilabs k.s. laboratory. Statistical evaluation led to the detection of common presence of Ureaplasmas and Mycoplasmas with some other microorganisms inhabiting the fiale urogenital tract. For the statistical part, I used the results of the patients for the period 1/1/2019 - 12/31/2022. I had a total of 859 results available. Based on them, I found out that the largest number of positive findings predominates Ureaplasma urealyticum, followed by a mixed infection of Ureaplasma urealyticum and Mycoplasma hominis to a lesser extent. Only in small number have been found positive Mycoplasma hominis. Among the associated microorganisms, the most frequent occurrence of lactobacilli was associated with Ureaplasma urealyticum positivity. Gardnerella vaginalis was found in all positivities with mycoplasmas, the highest in Ureaplasma urealyticum. Furthermore, Streptococcus anginosus and Candida albicans are represented to a similar extent, again to the greatest extent represented in the case of Ureaplasma urealyticum positivity. Other microorganisms were represented to a lesser extent and are described in more detail in the results of the thesis.
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Rod Lactobacillus a jeho význam v potravinářství
Olbertová (roz. Groligová), Žaneta
This thesis characterizes the genus Lactobacillus, one of a series of lactic acid bacteria. It specifies the bacteria significant species in the food industry. Furthermore, the thesis deals with the occurrence and importance of lactobacilli, mainly in food but also in relation to the human organism, animals and plant materials. The thesis describes the positive influence of lactobacilli on the human organism. A negative impact is described too, primarily from the perspective of food spoilage. In the conclusion of the thesis the practical part focuses mainly on the determination of this genus in raw goat's and cow's milk and in acidified milk.
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Characterization of bifidobacteria and lactobacilli isolated from intestinal tract of wild pigs
Šimurka, Julius ; Rada, Vojtěch (advisor) ; Švejstil, Roman (referee)
The microbiota of the gastrointestinal tract is considered as "acuqrated organ" located in a host organism. There are many performs of specific functions. The microbiota composition changes during the life of the individual, depending on his environment and nutrition. The process of identification and characterization of the bacteria from the intestinal microbiota is an important prerequisite for understanding the function of this "acuqrated organ".
This work deals with the anatomy and physiology of the digestive tract (DT) of pigs and evolutionary and eating differences of wild and domesticated pig, for understanding the natural environment of bacteria from DT, which in turn affects the bacterial diversity of the intestinal microbiota. Also mentioned is the process of domestication, which influenced the present form of domestic pig. The work includes the characteristics of lactobacilli and bifidobacteria, which contains the descriptions, taxonomy, metabolism and habitats. The work also shows the insulating media used in practice for the isolation of bifidobacteria and lactobacilli from DT of pigs and methods of their identification and related methods of characterization.
The available literature confirms the differences in the composition of microbiota DT between wild and domesticated pig, that correlate with other studies which study microbiota diversity of other domesticated and wild animals. The diversity studies of humans microbiota, also confirms the broad spectrum of bacterial species in primitive tribes of humans compared with urbanized man.
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Identification of selected probiotic bacteria in food additives
Bubeníková, Lucia ; Španová, Alena (referee) ; Rittich, Bohuslav (advisor)
Bifidobacteria and lactobacilli are gram-positive bacteria which belong to the lactic acid bacteria group. They are ordinary constituents of gastrointestinal microflora, play a pivotal role in human nutrition and health, prevent pathogen colonization and maintain normal mucosal immunity. In present days they are used in production of functional food products and pharmaceutical additives. Polymerase chain reaction (PCR) is used to the detection and identification of bacteria which belong to the genus of Bifidobacterium and Lactobacillus. Specific primers for each genus are used in PCR reactions for in vitro amplification of definite part of DNA. In this work, total DNA was isolated from three additives (Pangamin Bifi plus, Biopron Junior and Probiodom) by the method of phenol extraction and amplified using genus-specific PCR primers giving amplicons of 523 bp (Bifidobacterium) and 250 bp (Lactobacillus). Occurrence of bacteria of both genera was proved in all of the three tested probiotic products.
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