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Study of Production and Using Mesolactide
Gažo, Peter ; Porubský, Tomáš (referee) ; Petrůj, Jaroslav (advisor)
This bachelor thesis deals with mesolactide production of D,L-lactic acid. In the theoretical part, it has been processed literature review focused on manufacturing methods of mesolactide,it´s processing and using. In the practical part, it has been investigated conditions linked to the most appropriate production of mesolactide.
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Thermotolerant strains application for preparation of selected metabolites on waste hydrolysates
Musilová, Kristína ; Vítová, Eva (referee) ; Babák, Libor (advisor)
This Bachelor´s Thesis deals with the preparation of the selected metabolite on hydrolysates of orange fruit waste, specifically on orange peel hydrolysates using thermotolerant strains. The thermotolerant Bacillus coagulans strain having lactic acid as the primary metabolite, a substance with a great perspective, was selected for the fermentation process for its exceptional properties. At the beginning of the experiment, optimal conditions for the growth of the selected microorganism were determined; these were applied during the fermentations. Several fermentations were performed under various conditions until those conditions were determined, in which lactic acid was produced. The fermentation production was determined by the HPLC method and the saccharide decrease by the Somogyi-Nelson method. To achieve lactic acid production, it was necessary to extract the orange peels by an organic agent before fermentation, in order to eliminate substances causing inhibition of micro-organism growth. Higher yields were achieved by the SHF method, but the highest by using the Lactobacillus casei strain.
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The Study of Ethyllactate Synthesis from Lactic Acide Salts
Hlavatá, Daniela ; Figalla, Silvestr (referee) ; Petrůj, Jaroslav (advisor)
This diploma thesis deals with senthesis of lactic acid ethyl ester from slats of lactic acid. The literature review has been processed in the theoretical part of the diploma thesis. It is focused on manufacturing technologies of lactic acid esters, mainly on ethyl lactate by conventional and non-conventional ways of producing. Also the literature review involves producing of lactic acid and slats of lactic acid by fermentation because subsequently is possible produce ethyl lactate from that two substances. The laboratory apparatus for preparation of ethyl lactate and methyl lactate has been designed, realized and verificated function of it in the experimental part. There were carried out laboratory experimnets for production ethyl lactate and methyl lactate in this apparaturs.
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Thermotolerant strains application for preparation of selected metabolites on waste hydrolysates
Musilová, Kristína ; Vítová, Eva (referee) ; Babák, Libor (advisor)
This Bachelor´s Thesis deals with the preparation of the selected metabolite on hydrolysates of orange fruit waste, specifically on orange peel hydrolysates using thermotolerant strains. The thermotolerant Bacillus coagulans strain having lactic acid as the primary metabolite, a substance with a great perspective, was selected for the fermentation process for its exceptional properties. At the beginning of the experiment, optimal conditions for the growth of the selected microorganism were determined; these were applied during the fermentations. Several fermentations were performed under various conditions until those conditions were determined, in which lactic acid was produced. The fermentation production was determined by the HPLC method and the saccharide decrease by the Somogyi-Nelson method. To achieve lactic acid production, it was necessary to extract the orange peels by an organic agent before fermentation, in order to eliminate substances causing inhibition of micro-organism growth. Higher yields were achieved by the SHF method, but the highest by using the Lactobacillus casei strain.
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|
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Study of Production and Using Mesolactide
Gažo, Peter ; Porubský, Tomáš (referee) ; Petrůj, Jaroslav (advisor)
This bachelor thesis deals with mesolactide production of D,L-lactic acid. In the theoretical part, it has been processed literature review focused on manufacturing methods of mesolactide,it´s processing and using. In the practical part, it has been investigated conditions linked to the most appropriate production of mesolactide.
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|
|
The Study of Ethyllactate Synthesis from Lactic Acide Salts
Hlavatá, Daniela ; Figalla, Silvestr (referee) ; Petrůj, Jaroslav (advisor)
This diploma thesis deals with senthesis of lactic acid ethyl ester from slats of lactic acid. The literature review has been processed in the theoretical part of the diploma thesis. It is focused on manufacturing technologies of lactic acid esters, mainly on ethyl lactate by conventional and non-conventional ways of producing. Also the literature review involves producing of lactic acid and slats of lactic acid by fermentation because subsequently is possible produce ethyl lactate from that two substances. The laboratory apparatus for preparation of ethyl lactate and methyl lactate has been designed, realized and verificated function of it in the experimental part. There were carried out laboratory experimnets for production ethyl lactate and methyl lactate in this apparaturs.
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