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Vliv přídavku quercetinu na výskyt kokcidií u králíků
Dvořáčková, Nikola
This thesis investigated the effect of the flavonoid quercetin on the coccidiosis oocyst counts in rabbits. At the same time, it also investigated the impact on weight, daily gains, feed conversation ratio, daily and total consumption, and biochemical blood parameters. The experiment lasted a total of 116 days. Two groups of six piece weaned rabbits each of the Japanese rabbit breed were created. The age of the rabbits was 35–149 days in the experimental group and 31–145 days in the control group. The average weight of rabbits at the beginning of the experiment was 267 g, and at the end, it was 2288 g. A special feed mixture containing 1.9% quercetin was prepa-red for the experimental group. The mixture was added in the amounts of 1, 2, and 4 g per day, depending on their equal weight. The addition of 0.01 – 0.04 g/rabbit/day of the flavonoid quercetin did not have a negative effect on the health status, performance parameters, and biochemical para-meters of the blood. The experimental group had a lower average number of oocysts (EPG) than the control group (quercetin = 328, control = 640). There was a statisti-cally significant effect of quercetin on the number of coccidia oocysts (P < 0.05), pro-bably through its antioxidant effect, supporting immune reactions, and thereby main-taining a healthy intestinal environment. Quercetin can be a potential substance for the prevention of coccidiosis.
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Nemoci králíků a jejich prevence
Kostková, Jana
This Bachelor thesis with topic of Rabbit Diseases and Prevention starts with the history of rabbit domestication, brief description of its anatomy and its taxonomic rank. Its main focus was to descript the most substantial viral, bacterial, parasitic and gastrointestinal diseases, which appear the most in domestic breeding. As significant viral diseases we have chosen myxomatosis and rabbit plague. Chosen bacterial diseases are pasteurellosis, rabbit rhinitis, colibacillosis and rabbit spirochetosis. Coccidiosis and Toxoplasmosis were selected among parasitic diseases. For every disease, characteristic, etiology, pathogenesis, symptomatology, diagnostic and prevention were listed. The end of the thesis was dedicated to prevention of disease, breeding hygiene and sanitation.
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Application of molecular and cellular biology methods in research of protozoa Eimeria
Vrba, Vladimír ; Škvor, Jiří (advisor) ; Čepička, Ivan (referee) ; Hampl, Vladimír (referee)
Eimeria is an apicomplexan parasite causing disease coccidiosis that is most prominent in poultry farming industry. This thesis is aimed to develop new molecular tools and resolve issues that would be a valuable contribution in the field from both research and industry perspective. Because immunity to Eimeria is strictly species- specific, it is important to know and recognize correctly all species that parasitize the host. Traditional diagnostic approaches rely on classical methods such as oocyst morphology determination under the microscope, measurement of prepatent period or in-vivo assessment of lesions caused by this parasite. However, diagnostics of individual species using these methods is very time-consuming and it is often unreliable, especially when mixture of multiple species whose parameters overlap is analyzed. Methods utilizing conventional PCR to distinguish species already exist, however, they lack advantages offered by quantitative real-time PCR (qPCR). The first aim of this thesis was to develop qPCR assays for detection and quantification of seven Eimeria species which infect chicken utilizing single-copy non-polymorphic targets in order to ensure maximal specifity and coverage of all strains of each species. Usefulness of this method was demonstrated by analysis of field...
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