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Therapeutic use of alternative protein binders targeting tumor biomarkers in clinical testing of oncology patients
Tauš, Petr ; Drbal, Karel (advisor) ; Lepšík, Martin (referee)
Almost until the end of the last century, antibodies (aka immunoglobulins) were considered the only class of specific binding proteins. The discovery of hybridoma technology in 1975 had enabled the production of monoclonal antibodies and after twenty years some of them have entered clinical practice. Meanwhile, the first non-immunoglobulin protein scaffold, in which new specific binding sites could be introduced was discovered. To date, many different alternative scaffolds have been described, but only a few of them are being further developed for diagnostics, therapeutics or tools in basic research. Since these structures are overcoming the drawbacks of immunoglobulin structure, which are big size, expensive production and difficult rational design, they have potential to replace and exceed them. In this bachelor's thesis all the alternative scaffolds in development are summarized. Moreover, their advancements in clinical trials are described and compared with approved therapeutics based on immunoglobulin structure.
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Therapeutic use of alternative protein binders targeting tumor biomarkers in clinical testing of oncology patients
Tauš, Petr ; Drbal, Karel (advisor) ; Lepšík, Martin (referee)
Almost until the end of the last century, antibodies (aka immunoglobulins) were considered the only class of specific binding proteins. The discovery of hybridoma technology in 1975 had enabled the production of monoclonal antibodies and after twenty years some of them have entered clinical practice. Meanwhile, the first non-immunoglobulin protein scaffold, in which new specific binding sites could be introduced was discovered. To date, many different alternative scaffolds have been described, but only a few of them are being further developed for diagnostics, therapeutics or tools in basic research. Since these structures are overcoming the drawbacks of immunoglobulin structure, which are big size, expensive production and difficult rational design, they have potential to replace and exceed them. In this bachelor's thesis all the alternative scaffolds in development are summarized. Moreover, their advancements in clinical trials are described and compared with approved therapeutics based on immunoglobulin structure.
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Multiple sclerosis cerebrospinal - clinical tests reliability assesment
Rýdlová, Zuzana ; Řasová, Kamila (advisor) ; Joachimová, Marie (referee)
The purpose of this thesis is to verify inter-rater reliability and internal consistency of clinical tests included in battery of tests. The battery is composed of 12 tests - Low-Contrast Letter Acuity, Nine Hole Peg Test, Timed 25 - Foot Walk, Paced Auditory Serial Addition, Motoricity Index, Modified Ashworth Scale, Berg Balance Scale and tests of postural reactions, knee hyperextension, tremor, dysdiadochokinesia and ataxia. We evaluated inter-rater reliability by measuring the equality between two sets of results obtained by two observers investigating independently the same set of nine patients. To this aim we used two statistical methods: Intraclass corelation coefficient and Bland-Atman's graphs. Internal consistency was examined by applying Cohen's alpha coefficient. Satisfactory inter-rater reliability was found at 10 tests, only Modified Ashworth Scale and ataxia assesment indicate lower value. Internal consistency is sufficient at 10 tests, only tremor assesment and Motoricity Index didn't fulfill our criteria. Powered by TCPDF (www.tcpdf.org)
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