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Preparation of laboratory exercise for school subject Ecology in Manufacturing
Balcárková, Lucie ; Vaněk, Jiří (referee) ; Bača, Petr (advisor)
The thesis deals with the design of laboratory task on selected topics and brings together theoretical knowledge needed to understand the principle and function of the proposed system. The theoretical design is elaborated from the point of view of physical principles, physical feasibility, electronic difficulty, user service, correctness of the system, and its economic aspect. Furthermore, the practical implementation of the chosen task is disscussed in detail, based on the collected theoretical knowledge, a comparison of the available options and the results of the measurements made.
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Anatomical study of shade and sun European beech leaf under elevated CO2.
Horská, Jana ; Lhotáková, Zuzana (advisor) ; Urban, Otmar (referee)
The present Master thesis focuses on evalution of CO2 concentration and irradiance on selected leaf anatomical parameters of European Beech (Common Beech) Fagus sylvatica L.. The process of photosynthesis is remarkably determined by numerous extrenal factors, among them by atmoshperic CO2 concentration and irradiance and is closely correlated with leaf anatomical parameters. One of these most important anatomical parameters affecting the net assimilation rate is an internal leaf surface, which corresponds to mesophyll area avialable for gas exhcange. Experimental material of the study was sampled from the leaves of juvenile trees of F. sylvatica planted in 2005 and growing under ambient (390 ppm, AC) and elevated (700 ppm, EC) CO2 concentrations on the experimental site of the Global Change Research Center AS CR at Bílý Kříž in the Beskydy Mountains. Sun and shade leaves were sampled from trees of both CO2 treatments in two seasons 3 years apart (2009 and 2012). To determine leaf anatomical parameters, the stereological methods were applied, which yield unbiased estimation of measured parameters, particularly the Fakir method for internal leaf surface determination. The EC effect was observed on the leaves sampled in 2009 only in the decrease of proportion of intercellular spaces in mesophyll. In...
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Preparation of laboratory exercise for school subject Ecology in Manufacturing
Balcárková, Lucie ; Vaněk, Jiří (referee) ; Bača, Petr (advisor)
The thesis deals with the design of laboratory task on selected topics and brings together theoretical knowledge needed to understand the principle and function of the proposed system. The theoretical design is elaborated from the point of view of physical principles, physical feasibility, electronic difficulty, user service, correctness of the system, and its economic aspect. Furthermore, the practical implementation of the chosen task is disscussed in detail, based on the collected theoretical knowledge, a comparison of the available options and the results of the measurements made.
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Anatomical study of shade and sun European beech leaf under elevated CO2.
Horská, Jana ; Lhotáková, Zuzana (advisor) ; Urban, Otmar (referee)
The present Master thesis focuses on evalution of CO2 concentration and irradiance on selected leaf anatomical parameters of European Beech (Common Beech) Fagus sylvatica L.. The process of photosynthesis is remarkably determined by numerous extrenal factors, among them by atmoshperic CO2 concentration and irradiance and is closely correlated with leaf anatomical parameters. One of these most important anatomical parameters affecting the net assimilation rate is an internal leaf surface, which corresponds to mesophyll area avialable for gas exhcange. Experimental material of the study was sampled from the leaves of juvenile trees of F. sylvatica planted in 2005 and growing under ambient (390 ppm, AC) and elevated (700 ppm, EC) CO2 concentrations on the experimental site of the Global Change Research Center AS CR at Bílý Kříž in the Beskydy Mountains. Sun and shade leaves were sampled from trees of both CO2 treatments in two seasons 3 years apart (2009 and 2012). To determine leaf anatomical parameters, the stereological methods were applied, which yield unbiased estimation of measured parameters, particularly the Fakir method for internal leaf surface determination. The EC effect was observed on the leaves sampled in 2009 only in the decrease of proportion of intercellular spaces in mesophyll. In...
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Mesophyll CO2 conductance in consequence with the leaf anatomy and environmental factors.
Malcová, Kateřina ; Lhotáková, Zuzana (advisor) ; Nátr, Lubomír (referee)
The CO2 diffusion pathway from the atmosphere surrounding the leaf to the chloroplast stroma is composed of several parts, where each part poses certain resistance to the CO2 flux. The CO2 diffusion from the sub-stomatal cavities to the stroma is limited due to mesophyll conductance (gm), which includes CO2 movement in gas, liquid and lipid phases. The mesophyll conductance is influenced by factors that are both internal (e.g. leaf anatomy and leaf age) and external (e.g. irradiance, temperature). Internal factors exhibit rather long-term responses of gm, while external factors are involved in short-term changes. For these rapid changes are probably responsible aquaporins. gm significantly influences the rate of net photosynthesis, almost in the same range as a stomatal conductance does. The limitation of photosynthesis given by mesophyll conductance is not fixed and varies among species and plant functional groups. Due to continual increase of CO2 concentration in the atmosphere, variability in gm can lead to enhanced competitive potencial of some plant species.
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The effect of elevated CO2 concentration and irradiation on quantitative parameters of mesophyll cells of Norway spruce
Kubínová, Zuzana ; Albrechtová, Jana (advisor) ; Radochová, Barbora (referee)
KUBÍNOVÁ, Zuzana. The effect of elevated CO2 concentration and irradiation on quantitative parameters of mesophyll cells of Norway spruce. Prague, 2010. 74 p. Master's degree thesis. Faculty of Science, Charles University in Prague. Abstract The aim of the present thesis was to choose and adjust a suitable methodology for counting particles in 3D space, which would be suitable for unbiased estimation of chloroplast number in needle mesophyll cells. The disector method was used for the first time to determine the number of chloroplasts. This method enables unbiased estimation of chloroplast number in needle volume from optical sections captured from fresh free-hand sections by confocal microscope. The sections did not need any pre-processing. Another aim was to compare selected photosynthetic and anatomical characteristics of sun and shade Norway spruce needles, which were grown under different CO2 concentration. The trees were grown for eight years in ambient (during the experiment increasing from 357 up to 370 µmol CO2 ∙ mol-1 ) CO2 concentration or elevated (700 µmol ∙ mol-1 ) CO2 concentration in special glass domes on an experimental research site of the Institute of Systems Biology and Ecology, Academy of Sciences of the Czech Republic at Bílý Kříž in Moravskoslezské Beskydy mountains. The sun needles...
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