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The skin immune response of mice infected with avian schistosomes
Revalová, Alena ; Macháček, Tomáš (advisor) ; Kolářová, Libuše (referee)
Invasion of the mammalian skin by cercariae of avian schistosomes of the genus Trichobilharzia is associated with the skin pathology called cercarial dermatitis (CD). Although the manifestation of CD, characterized by the presence of maculo-papular rash and pruritus, is believed to be linked with the previous sensitization, the role of the infection dose and the specific causative agent is rarely considered. Therefore the skin immune response in mouse pinnae infected by 100, 1000 or 4x100 (repeated infections) cercariae of two Trichobilharzia species - T. regenti and T. szidati - was examined in this work. To compare the effect of these factors (the infection dose and species), a complex approach was ensured by employing both in vivo and in vitro methods. Firstly, histopathological changes in the pinnae were examined by classical histology and infiltrated leukocytes were characterized by flow cytometry. Sera of mice were used to detect the systemic production of cytokines and parasite-specific antibodies. In vitro cultivation of mouse pinnae was used to detect the local production of cytokines. The most remarkable observation was that the infection dose largely impacted the course of the immune response, both locally and systematically. In primary infected mouse pinnae, 100 cercariae of both...
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MAJOR EPIDEMIOLOGICAL DISEASES AND POSSIBILITIES OF RESTRICTION ITS MALFEASANCE AND CONSEQUENTIAL SPREAD IN POPULATION
VAŇOVÁ, Naďa
In history there are cases of epidemics, which often reduced the human population more than war conflicts ever did. A typical example is the Spanish flu epidemic during the 1st World War. It is generally known that a large portion of our population lacks sufficient knowledge on the very dangerous diseases, which can be misused by bioterrorists. People underestimate the threat and often they don?t even have any idea how to protect themselves against biological agents or what to do in the event of a possible attack. I have chosen this issue as the topic of my diploma work as I consider the current situation of the inhabitant?s awareness of this issue as unsatisfactory. The objective of my work is to define epidemiologically serious diseases, which are suitable for misuse within the bioterrorists? actions, to focus on their diagnostics, treatment and prevention. On the basis of studying expert literature and consultations I have proposed preventative measures and instructions what to do in the event of a possible risk of attack by biological warfare agents and subsequently I created two types of information leaflets for the wide public. At the same time I handed over these two information leaflets for an expert assessment to a legal office to make sure that they do not fulfil the state of facts of a criminal offence of spreading a haux. The result is an opinion that the leaflet with general information may be used to increase peoples' awareness on the preventative protection against the possible risk of attack or misuse of biological agents. The second leaflet with the detailed information may only be used for the purpose of this diploma work, or possibly for training purposes of cooperation of the Integrated Emergency Services and the inhabitants in the given area.
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