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Monitoring of the wheat quality for food production
Slavíčková, Radka ; Hýsková, Eva (referee) ; Omelková, Jiřina (advisor)
The aim of this study was to monitor the quality of wheat Triticum aestivum, imported into the laboratory MORAGRO after harvest by eight different suppliers, and to evaluate the main quality parameters decisive for the final use of cereal crops. The introduction of the theoretical part was devoted to understanding commercial and agricultural importance of wheat and description of morphological and physiological characteristics of wheat grain. The core of the theoretical part has been focused on the description and explanation of physical and chemical properties of the wheat grain, which largely affect the final product quality of wheat. External factors affecting final quality and yields of wheat were also commented. The experimental part was based on measurements of main quality parameters of wheat (moisture content, bulk density, falling number, amount of gluten and the wheat proteins, sedimentation value and content of additives and impurities). In most cases, measurements were carried out using automated instruments. The quality of wheat was determined based on the obtained results, which allows deciding, whether it is appropriating for food production.
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Use of liquid chromatography in pharmaceutical analysis and preparation of monolithic stationary phases for thin-layer chromatography
Vojta, Jiří ; Coufal, Pavel (advisor) ; Tůma, Petr (referee) ; Jelínek, Ivan (referee)
(EN) In the first part of this work, analytical methods for determination of impurities of active pharmaceutical ingredients (API) in combined pharmaceutical dosage forms were developed and validated. Development of the methods covered both the optimization of sample preparation procedure and chromatographic conditions. The methods were validated according to International Conference on Harmonization guideline and both of them were confirmed to be able to analyze stability samples. Impurities in paracetamol, codeine phosphate hemihydrate and pitophenone hydrochloride in the presence of fourth API fenpiverinium bromide were separated by using ion-pair reversed phase chromatography with gradient elution. Symmetry C18, 250 x 4,6 mm, 5 µm heated to 35 řC was used as a separation column. A diode array detector was used. The detection wavelengths were set as follows: 220 nm for paracetamol impurity K, 245 nm for paracetamol and its other impurities and 285 nm for codeine, pitophenone and their impurities. Impurities in valsartan, amlodipine besylate and hydrochlorothiazide were separated by reversed phase UHPLC method with gradient elution. Chromatographic column Zorbax Eclipse C8 RRHD, 100 x 3,0 mm, 1,8 µm heated to 30 řC and spectrophotometric detection were used. The detection wavelengths were set as...
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Electronic structure and magnetic properties of the materials with strong electron-electron correlation
Kozub, Agnieszka Lidia ; Shick, Alexander (advisor) ; Legut, Dominik (referee) ; Minár, Ján (referee)
In this thesis, we summarize the material-specific theories of strongly correlated systems and apply them to selected materials. We describe and apply the corre- lated band theory methods: the local density approximation plus Coulomb U, and the density functional theory plus exact diagonalization of single impurity An- derson model. First, we investigate the systems containing impurity atoms: cobalt impurity located in the bulk copper and samarium, and neodymium adatoms on the surface of graphene. We present the spectral densities and study the magnetism of those compounds. Afterwards, we analyze three Np-based compounds: NpPt2In7, Np2Ni17 and NpBC. For all three compounds we analyze the spin, orbital and to- tal magnetic moments and the total density of states, as well as its projections for selected orbitals and spins. Moreover, for NpPt2In7 and NpBC we perform the to- tal energy analysis between different magnetic moment arrangements on the Np atoms.
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Use of liquid chromatography in pharmaceutical analysis and preparation of monolithic stationary phases for thin-layer chromatography
Vojta, Jiří
(EN) In the first part of this work, analytical methods for determination of impurities of active pharmaceutical ingredients (API) in combined pharmaceutical dosage forms were developed and validated. Development of the methods covered both the optimization of sample preparation procedure and chromatographic conditions. The methods were validated according to International Conference on Harmonization guideline and both of them were confirmed to be able to analyze stability samples. Impurities in paracetamol, codeine phosphate hemihydrate and pitophenone hydrochloride in the presence of fourth API fenpiverinium bromide were separated by using ion-pair reversed phase chromatography with gradient elution. Symmetry C18, 250 x 4,6 mm, 5 µm heated to 35 řC was used as a separation column. A diode array detector was used. The detection wavelengths were set as follows: 220 nm for paracetamol impurity K, 245 nm for paracetamol and its other impurities and 285 nm for codeine, pitophenone and their impurities. Impurities in valsartan, amlodipine besylate and hydrochlorothiazide were separated by reversed phase UHPLC method with gradient elution. Chromatographic column Zorbax Eclipse C8 RRHD, 100 x 3,0 mm, 1,8 µm heated to 30 řC and spectrophotometric detection were used. The detection wavelengths were set as...
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Development of an UHPLC method for stability study of venetoclax
Máchalová, Jitka ; Křížek, Tomáš (advisor) ; Kozlík, Petr (referee)
Tato práce se zabývá vývojem chromatografické metody pro stanovení venetoclaxu a kontrolu jeho čistoty pomocí techniky UHPLC. Venetoclax je nízkomolekulární inhi- bitor antiapoptického proteinu Bcl-2, který hraje důležitou roli při regulaci apoptózy a při vzniku hematologické malignace. Venetoclax se tedy používá pro léčbu chronické lymfocytární leukemie. Kvůli problematické rozpustnosti analytu byl jako rozpouště- dlo vzorku zvolen 10mM vodný roztok H3PO4 : ACN s 40% objemovým zastoupením acetonitrilu. Dále byla nalezena optimální kombinace stacionární a mobilní fáze. Optimalizace byla prováděna především s ohledem na faktor chvostování píku vene- toclaxu a jeho rozlišení od nečistoty eluující v jeho těsné blízkosti. V optimalizované metodě byla použita kolona Acquity UPLC BEH Phenyl (100 × 2,10 mm; 1,7 µm) a jako mobilní fáze směs pufru (10mM H3PO4/NH3 o pH 2,5) s přídavkem 0,5% trifluoroctové kyseliny a ACN. Součástí validace metody bylo testování přesnosti, opa- kovatelnosti, meze detekce a kvantifikace, linearity a lineárního dynamického rozsahu, robustnosti metody a testování stability vzorku. Opakovatelnost retenčního času byla 0,07 % a opakovatelnost plochy píku byla 0,45 %. Hodnota meze detekce metody byla 1,6 · 10−5 mg mm−1 a hodnota meze kvantifikace byla 5,4 · 10−5 mg mm−1 . Linearita...
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Electronic structure and magnetic properties of the materials with strong electron-electron correlation
Kozub, Agnieszka Lidia ; Shick, Alexander (advisor) ; Legut, Dominik (referee) ; Minár, Ján (referee)
In this thesis, we summarize the material-specific theories of strongly correlated systems and apply them to selected materials. We describe and apply the corre- lated band theory methods: the local density approximation plus Coulomb U, and the density functional theory plus exact diagonalization of single impurity An- derson model. First, we investigate the systems containing impurity atoms: cobalt impurity located in the bulk copper and samarium, and neodymium adatoms on the surface of graphene. We present the spectral densities and study the magnetism of those compounds. Afterwards, we analyze three Np-based compounds: NpPt2In7, Np2Ni17 and NpBC. For all three compounds we analyze the spin, orbital and to- tal magnetic moments and the total density of states, as well as its projections for selected orbitals and spins. Moreover, for NpPt2In7 and NpBC we perform the to- tal energy analysis between different magnetic moment arrangements on the Np atoms.
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Use of liquid chromatography in pharmaceutical analysis and preparation of monolithic stationary phases for thin-layer chromatography
Vojta, Jiří ; Coufal, Pavel (advisor) ; Tůma, Petr (referee) ; Jelínek, Ivan (referee)
(EN) In the first part of this work, analytical methods for determination of impurities of active pharmaceutical ingredients (API) in combined pharmaceutical dosage forms were developed and validated. Development of the methods covered both the optimization of sample preparation procedure and chromatographic conditions. The methods were validated according to International Conference on Harmonization guideline and both of them were confirmed to be able to analyze stability samples. Impurities in paracetamol, codeine phosphate hemihydrate and pitophenone hydrochloride in the presence of fourth API fenpiverinium bromide were separated by using ion-pair reversed phase chromatography with gradient elution. Symmetry C18, 250 x 4,6 mm, 5 µm heated to 35 řC was used as a separation column. A diode array detector was used. The detection wavelengths were set as follows: 220 nm for paracetamol impurity K, 245 nm for paracetamol and its other impurities and 285 nm for codeine, pitophenone and their impurities. Impurities in valsartan, amlodipine besylate and hydrochlorothiazide were separated by reversed phase UHPLC method with gradient elution. Chromatographic column Zorbax Eclipse C8 RRHD, 100 x 3,0 mm, 1,8 µm heated to 30 řC and spectrophotometric detection were used. The detection wavelengths were set as...
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Use of liquid chromatography in pharmaceutical analysis and preparation of monolithic stationary phases for thin-layer chromatography
Vojta, Jiří
(EN) In the first part of this work, analytical methods for determination of impurities of active pharmaceutical ingredients (API) in combined pharmaceutical dosage forms were developed and validated. Development of the methods covered both the optimization of sample preparation procedure and chromatographic conditions. The methods were validated according to International Conference on Harmonization guideline and both of them were confirmed to be able to analyze stability samples. Impurities in paracetamol, codeine phosphate hemihydrate and pitophenone hydrochloride in the presence of fourth API fenpiverinium bromide were separated by using ion-pair reversed phase chromatography with gradient elution. Symmetry C18, 250 x 4,6 mm, 5 µm heated to 35 řC was used as a separation column. A diode array detector was used. The detection wavelengths were set as follows: 220 nm for paracetamol impurity K, 245 nm for paracetamol and its other impurities and 285 nm for codeine, pitophenone and their impurities. Impurities in valsartan, amlodipine besylate and hydrochlorothiazide were separated by reversed phase UHPLC method with gradient elution. Chromatographic column Zorbax Eclipse C8 RRHD, 100 x 3,0 mm, 1,8 µm heated to 30 řC and spectrophotometric detection were used. The detection wavelengths were set as...
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Identification of ropinirole and its impurities by mass spectrometry
Vaňkátová, Petra ; Coufal, Pavel (advisor) ; Čabala, Radomír (referee)
This thesis is focused on the analysis of ropinirole and its impurities related to the synthesis process using mass spectrometry. The theoretical introduction summarizes method of mass spectrometry. The experimental part is devoted to interpretation of mass spectra of samples measured both without fragmentation and using the CID fragmentation. Measurements were performed on a triple quadrupole with electrospray ionization. Properties of samples with respect to the quantification using SRM mode are also summarized in the paper. The main contribution of this work is clarification of the fragmentation of ropinirole and its impurities, which may serve for further studies of these substances. Powered by TCPDF (www.tcpdf.org)
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Monitoring of the wheat quality for food production
Slavíčková, Radka ; Hýsková, Eva (referee) ; Omelková, Jiřina (advisor)
The aim of this study was to monitor the quality of wheat Triticum aestivum, imported into the laboratory MORAGRO after harvest by eight different suppliers, and to evaluate the main quality parameters decisive for the final use of cereal crops. The introduction of the theoretical part was devoted to understanding commercial and agricultural importance of wheat and description of morphological and physiological characteristics of wheat grain. The core of the theoretical part has been focused on the description and explanation of physical and chemical properties of the wheat grain, which largely affect the final product quality of wheat. External factors affecting final quality and yields of wheat were also commented. The experimental part was based on measurements of main quality parameters of wheat (moisture content, bulk density, falling number, amount of gluten and the wheat proteins, sedimentation value and content of additives and impurities). In most cases, measurements were carried out using automated instruments. The quality of wheat was determined based on the obtained results, which allows deciding, whether it is appropriating for food production.
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