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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Testing and optimization of protocols for removal of contaminants during DNA isolation from hibiscus blossoms
 Brabcová, Martina ; Kubalová, Michaela (referee) ; Fialová, Lenka (advisor)
The Roselle (Hibiscus sabdariffa) is a widely used plant in food industry, especially in tea production. The hibiscus flower is rich in polyphenolic substances, which are a major problem in DNA isolation. This work deals with the modification of the CTAB isolation protocol to ensure concentration and purity of DNA sufficient for its amplification. DNA isolated according to the CTAB protocol did not have sufficient concentration and purity for amplification due to high contamination with polyphenolic substances. A procedure with double incubation of plant tissue in CTAB buffer containing 3% polyvinylpyrrolidone (PVP) was proposed to remove it. These isolates were successfully amplified. The effect of PVP concentration (1-5%) in CTAB buffer on the concentration and purity of isolated DNA was also investigated. PCR results showed that double incubation with CTAB buffer had a greater effect on the removal of polyphenolic compounds than changing the PVP concentration. Subsequently, the modified isolation protocol was also successfully used to isolate amplifiable DNA from commercial tea blends.
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Determination of aluminium concentration changes in infusion and digesta of Hibiscus sabdariffa
 Binderová, Denisa ; Potůčková, Miroslava (advisor) ; Blanka, Blanka (referee)
The aim of this bachelor thesis was to characterize aluminium content in infusion and digestate of Hibiscus sabdariffa tea. Dried Hibiscus is worldwide used for the manufacture of herbal or fruit tea. Aluminium is at excessive exposure toxic for human organism. The amount of this element which is human gastrointestinal tract able to intake and absorb depends on many factors such as the form of it, physico-chemical properties of a food matrix and on their behavior in the human gastrointestinal system. The concentration of aluminum in the dried plant matrix is influenced bythe ability of plant to accumulate element from the soil and create aluminium complexes in tissues. This process protects plant against aluminium toxicity. In experimental part of bachelor thesis was determined the amount of aluminum in dried plant matrix of 8 herbal teas of hibiscus flower, their infusions and digestates by emission spectrometry with inductively coupled plasma (ICP-OES). Digestates from infusions were prepared according to the current version of the static in vitro gastrointestinal model Infogest Working Group. The content of the element in plant matrix was not (p < 0.05) effect by the type of agricultural production (conventional × ecological) or country of origin. From dried hibiscus flowers passed only cca 5 % of aluminium into the infusion and this amount was not (p < 0.05) proportional to the initial content of element. There was a statistically significant (p > 0.05) increase in the concentration of aluminium after infusions digestion via simulated gastrointestinal process. The observed increase of aluminum content was probably caused by the acidic pH of the gastric phase of digestion where aluminium passed into well soluble and therefore better absorbable forms.
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