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Vliv změny stravovacího režimu na zastoupení vybraných druhů rodu Bifidobacterium v lidském trávicím traktu
Vanišová, Denisa
The diploma thesis provides insight into the influence of dietary interventions on the composition of the human intestinal microbiota. Focusing on the representation of bacteria belonging to the genera Bacteroides, Eubacterium and Bifidobacterium, with an emphasis on the identification and percentage representation of B. longum and B. bifidum using the PCR method. The research involved 12 participants who took part in a group weight reduction programme over the course of 8 weeks. Stool samples were analysed before starting and after finishing the course. It was ascertained that during the course there was a significant increase in the number of bacteria of the genus Bifidobacterium, whereas in the genus Bacteroides there was observed a concurrent decrease. Regarding Eubacterium spp. there was almost no observed difference. Over the course of the programme subjects significantly increased their fibre intake, while decreasing intake of all other macronutrients. No correlation was confirmed between the numbers of bifidobacteria and the amount of nutrients consumed.
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Human gut microbiome: Origin, ontogenetic development, diversity and its use in anthropology
Dvořáková, Barbora ; Daňková, Pavlína (advisor) ; Schierová, Dagmar (referee)
Human gut microbiome is a broad term encompassing all microorganismal life inhabiting the human gut. The bacteria living in the human gut represents the largest group of the human microbiome, make up the significant percentage of the human cellular composition and their genomes comprise a big part of the human genome. Gut microbiome has a significant role in human health and changes throughout the human life in reaction e.g. to change of diet and medical drug usage. This work pursues the acquisition and development of gut microbiome, the factors influencing its formation and diversity, and its use in anthropology.
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The effect of gluten-free diet on β-cell residual capacity, immune function and gut microbiome in children with newly diagnosed type 1. diabetes
Neuman, Vít ; Šumník, Zdeněk (advisor) ; Pelikánová, Terezie (referee) ; Škvor, Jaroslav (referee)
The effect of gluten-free diet on β-cell residual capacity, immune function and gut microbiome in children with newly diagnosed type 1. diabetes Abstract The pathophysiology of the onset and progression of type 1 diabetes (T1D) is not fully understood. Gluten has a proinflammatory effect on the immune system and is therefore considered as one of the factors affecting the onset and progression of T1D. The aim of the thesis is to allow a complex insight into the role of the GFD on the residual β-cell capacity, T1D control, gut microbiome, gut permeability, subtypes of immune cells and the effect of gut microbiome transfer into germ-free non-obese diabetic (NOD) mice on the incidence of diabetes. On the group of 45 children with T1D (26 intervention group, 19 control group) we proved the association of the GFD with slower decrease of β-cell residual capacity (the difference in the trend of C-peptide decrease 409 pmol/l/year; p = 0,04) and lower HbA1c (by 7,8 mmol/mol; p=0,02). We also described the changes in the gut bacteria that were differentially abundant after the administration of the GFD and the changes in abundance of the regulatory and effector immune cells. We showed there was no change in the gut permeability with respect to the study group. We also proved that the transfer of human gut microbiota...
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Human gut microbiome: Origin, ontogenetic development, diversity and its use in anthropology
Dvořáková, Barbora ; Daňková, Pavlína (advisor) ; Schierová, Dagmar (referee)
Human gut microbiome is a broad term encompassing all microorganismal life inhabiting the human gut. The bacteria living in the human gut represents the largest group of the human microbiome, make up the significant percentage of the human cellular composition and their genomes comprise a big part of the human genome. Gut microbiome has a significant role in human health and changes throughout the human life in reaction e.g. to change of diet and medical drug usage. This work pursues the acquisition and development of gut microbiome, the factors influencing its formation and diversity, and its use in anthropology.
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