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Oncoretroviruses: tumor transformation mechanisms of hematopoietic cells resulting in leukaemia
Gašpareková, Mária ; Bendová, Michaela (advisor) ; Mrvová, Silvia (referee)
HTLV-1 and FeLV are retroviruses, which are able to transform host cells and cause cancer, mostly leukemia, in infected organism. Belonging to Retroviridae family and both using very similar genome, these viruses developed different ways to reach transformation of infected cells. While FeLV uses insertional activation close to cellular proto-oncogenes in order to regulate transcription of these genes or carries cellular oncogene in its genome, HTLV-1 codes viral proteins which are able to regulate many processes of the cell. One of these proteins is Tax, which regulates many events in the cell, such as signalization, cell cycle, apoptosis and others. Another protein responsible for oncogenesis is HBZ, which is transcribed from antisense strand of proviral DNA. In the end HTLV-1 and FeLV strategies causing cancer are compared with some other leukemic retroviruses in order to show, that molecular strategies described on examples of HTLV-1 and FeLV are more or less common also for other oncogenic retroviruses. Key words: HTLV-1, FeLV, transformation, leukaemia, tumor, oncogene
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Public awareness of the issue of donating bone marrow
Buriánková, Hana ; Sojková, Monika (advisor) ; Klamová, Hana (referee)
The primary aim of this diploma thesis was to draw attention to the issue of bone marrow donation. The thesis focuses on the level of public awareness and people's significant doubts regarding bone marrow donation, as these doubts and apprehensions might be the main reason restraining them from entering the bone marrow donor register. The theoretical part provides a comprehensive summary of findings in this area. Substantial part addresses particular types of the hematopoietic cells taking process and the following donor treatment. The empirical part of this diploma thesis constitutes of a quantitative research, in which hundereds of respondents have been reached through an electronic questionnaire. Within exactly 10 days, 576 of them filed for the survey, 496 of them were valid and processed. The outcome of the research reflects strong need for raising factual public awareness in the area of hematopoietic cells donation, especially in respect to current donation methods. 79,51 % of respondents had no knowledge of peripheral blood stem cell (PBSC) donation method. 50,26 % of them admitted fear of the bone marrow donation procedure itself. Respondents taking part in the research showed great interest in the topic. 85,48 % of them would appreciate further education and 82,66 % assumes that...
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