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National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Vliv vybraných těžkých kovů na fyziologii ryb
 Šanda, Radek
The study aims on effects of mercury on haematological and biochemical parameters in blood plasma on common carp (Cyprinus carpio L.). The theoretical part of study is focused on phenomenon of occurrence of mercury and its forms in aquatic environment and fish. Practical portion of study includes the effects of administration to mercury and also the effects of co-administration of sodium chloride. In the first experiment we carried out the exposure of Cyprinus carpio L. by selected mercury concentrations for 5, 10 and 15 days. In the second experiments were carps exposed to 1.5 micro g.l-1 mercury with the addition of various concentrations of sodium chloride (29 mg.l-1, 300 mg.l-1 and 1000 mg.l-1) for 72 h. For the evaluation of haematological and biochemical biomarkers in blood plasma of carps, we exploited the Konelab T 20xt and Easy Lyte machines. The obtained data were statistically processed and evaluated.
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Vliv vybraných těžkých kovů na fyziologii ryb
 Šanda, Radek
The study aims on effects of mercury on haematological and biochemical parameters in blood plasma on common carp (Cyprinus carpio L.). The theoretical part of study is focused on phenomenon of occurrence of mercury and its forms in aquatic environment and fish. Practical portion of study includes the effects of administration to mercury and also the effects of co-administration of sodium chloride. In the first experiment we carried out the exposure of Cyprinus carpio L. by selected mercury concentrations for 5, 10 and 15 days. In the second experiments were carps exposed to 1.5 micro g.l-1 mercury with the addition of various concentrations of sodium chloride (29 mg.l-1, 300 mg.l-1 and 1000 mg.l-1) for 72 h. For the evaluation of haematological and biochemical biomarkers in blood plasma of carps, we exploited the Konelab T 20xt and Easy Lyte machines. The obtained data were statistically processed and evaluated.
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Hematological changes in fish exposed to nitrites
 FRANĚK, Roman
The aim of the thesis was to determine the effect of nitrite on hematological indices of rainbow trout (Oncorhynchus mykiss) and Nile tilapia (Oreochromis niloticus). After the fish were exposed to various concentrations of nitrites were determined 48hLC50. For the main test only fractional concentrations of these values were used. For rainbow trout it was 8 mg.l-1 NO2?, for Nile tilapia 11 mg.l-1 NO2?. The exposure of both species lasted for 48 hours. From fish blood were made blood smears to determine the influence of nitrite on size changes of erythrocytes and hematological parameters were set also. The effect of nitrite on changes in the ultrastructure of erythrocytes was determined by the electron microscopy. The erythrocyte nuclei of rainbow trout showed significant (p <0.05) shrinkage. The images of the electron microscope showed an increased amount of changes, especially in the form of various units within the cytoplasm of erythrocytes, was also visible nuclei shrinkage. There was significant (p < 0.01) reduction in the amount of hemoglobin and significant (p < 0.01) increase in the concentration of methemoglobin levels of the exposed groups. In the number of erythrocytes and hematocrit were not detected any changes. Hematological parameters of Nile tilapia did not show any size changes in the measured parameters, no changes were detected in the count of erythrocytes, hemoglobin and hematocrit values.
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