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	Účinnost vybraných silic při ochraně bramboru vůči bakterii Pectobacterium atrosepticum Mokrá, Kristýna The aim of the thesis was to evaluate the effect of the active substances of essential oils (thyme, oregano, rosemary, natural thymol, synthetic thymol, carvacrol, menthon, cinnamon) against the Gram-negative bacterium Pectobacterium atrosepticum. The efficacy of essential oils was determined under laboratory conditions using the disk diffusion method, the values of minimum inhibitory concentration and minimum bactericidal concentration. Also, a pathogenicity test was performed on potato tubers with selected essential oils (thyme, thymol natural, and cinnamon). The components natural thymol, synthetic thymol, and the essential oil of cinnamon showed the best results, while menthone and carvacrol showed poor effects against the tested bacteria. Detailed record
	Metody dekontaminace rekombinantních proteinů od bakteriálního lipopolysacharidu CHARVÁTOVÁ, Lucie In this work, three decontamination methods based on two-phase micellar system and affinity chromatography were used to decontaminate recombinant proteins from bacterial lipopolysaccharide and to determine which method is the most effective. The efficiency of this method was measured using various recombinant proteins at several protein concentrations. Three different assays, two chromogenic and one fluorogenic, were used to measure the concentration of endotoxins in samples. The most accurate method for measuring the concentration of endotoxins was determined. Detailed record
	Membrane vesicles in bacteria and their role in antibiotic resistance Benešová, Anna ; Konopásek, Ivo (advisor) ; Pospíšil, Jiří (referee) Membrane vesicles are produced by both Gram-positive and Gram-negative bacteria. Mechanism of their formation differs between these two groups of bacteria. It is caused by the different structure of their cell envelope. Gram-negative bacteria contain outer membrane and membrane vesicles can originate from this membrane. Membrane vesicles of Gram-positive bacteria are derived from the cytoplasmic membrane. They have to cross the barrier of the thick layer of peptidoglycan. Membrane vesicles contain cellular components whose properties enable vesicles to fulfil various functions. Antibiotic resistance can be counted as one of these functions. This thesis discusses three ways used by the membrane vesicles to protect the cells from antibiotics: transport of enzymes that degrade antibiotics, removal of antibiotics from cell's surroundings and role of membrane vesicles in horizontal gene transfer. Detailed record

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