National Repository of Grey Literature 1 records found  Search took 0.01 seconds. 
Isolation, characterization and localization of orthologous sequence of the \kur{Drosophila Notch} gene in codling moth, \kur{Cydia pomonella}
KŮTA, Václav
The codling moth, Cydia pomonella (Tortricidae) is a significant pest in apple orchards. In the 1990ies, a control programme using the Sterile Insect Technique (SIT) has been established, based on bisexual releases of sterile insects into wild populations of this pest. Male-only releases are not possible due to the lack of an efficient system to produce male-only progeny. Recently, a new approach has been proposed for the development of genetic sexing strains in Lepidoptera. It is based on insertion of a dominant conditional lethal mutation of the Notch gene, derived from the N60g11 allele of Drosophila, into the female W chromosome by means of transgenesis. This study deals with isolation, characterization, and chromosomal localization of a codling moth orthologue of the Drosophila Notch gene with the aim to prepare a mutant sequence of the orthologue to be used in plasmid constructs for germline transformation of this pest.

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