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National Repository of Grey Literature 3 records found  Search took 0.01 seconds. 
Genetic engineering of \kur{psbA} gene in \kur{Nicotiana tabacum}
 HUCKOVÁ, Dagmar
Transformation vector with mutated psbA gene and selective aadA gene was created and transferred into Nicotiana tabacum living cells using Biolistic bombardment. Due to homologous recombination, transformed plant lineage carrying D1-A209, D1-C-212 instead of D1-S209, D1-S212 in D1 protein in PS II was obtained. Seeds from transformed plant were harvested and homoplasmy of the first generation was tested. These mutations caused higher thermostability in Synechocystis sp. PCC6803 so the transformed plant is expected to be the first step in the study of PS II thermostability in higher plants.
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Genetic transformation of embryogenic cultures of Norway spruce (\kur{Picea abies})
 KOLÁŘOVÁ, Klára
The aim of this study was to develop a transformation procedure for embryonic cultures of Norway spruce (Picea abies) using particle bombardment. Selection of the transgenic tissue was based on the antibiotic resistance conferring by the nptII gene, in combination with the antibiotic kanamycin. Expression of the gus gene was confirmed by histochemical analysis.
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Genetic transformation of ornamental plants
 Pavingerová, Daniela
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