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UTILIZATION OF ALTERNATIVE TESTS OF BIOTOXICITY FOR DETERMINATION INADVISABILITY OF EXISTING AND/OR POTENTIONAL POLUTANTS
Vašulková, Lucie ; Zlámalová Gargošová, Helena (referee) ; Bednařík, Karel (advisor)
This thesis is focused on attestation of ecotoxicity of octachlorstyrene (OCS), persistent organic pollutant (POP), by biological tests of toxicity. The toxicity of OCS was determined by using two kinds of alternative tests applied on fresh water organisms. First of them, Thamnotoxkit, is based on observation of mobility, immobilization or even killing of crustacean Thamnocephalus platyurus. The second one, Daphnotoxkit, applies crustacean Daphnia magna. For purposes of comparison, a standard test of toxicity – so called test of root growth inhibition of Sinapis alba and two fytotests – test of growth inhibition of Lemna minor and test of root growth inhibition of Allium cepa were performed as well.
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The effect of toxicity and othet parameters of substrate on plant growth in post mining sites.
Zadinová, Radka ; Frouz, Jan (advisor) ; Kočí, Vladimír (referee)
Surface mining of coal in the country leaves a lot of damage. This type of mining is characterized by the creation of large external dumps. The dumps often contain substrates with toxic substances or substances which become toxic after reaction with air. These substrates are toxic to plants and edaphon. This work is intended to show what properties of substrates are important for phytotoxicity. The location of research was selected including 3 different kinds of substrates, terrain unevenness and a lagoon in the middle. Coordinate grid has been created on the selected location. At each point a test was conducted with plant Sinapis alba and biomass was extracted from a naturally growing vegetation. Further, at the points substrates were collected and values of pH, conductivity and arsenic were measured. Test with plant Sinapis alba was repeated in the laboratory conditions as well. On site GPS coordinates were measured for the mathematical model created in ArcGIS. Experiment has shown the importance of the type of substrate and the geologic description, and then the association between dejection, value of pH and conductivity. Conductivity and pH levels also correlate with the germination Sinapis alba and biomass of naturally growing vegetation.
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The effect of artificial weathering on overburden properties
Zadinová, Radka ; Frouz, Jan (advisor) ; Bartuška, Martin (referee)
Surface mining of coal cause massive changes of landscape. Dumps, heaps and tailings created vast areas in post mining landscape. Soil formation is influenced by the weathering process. Sample-the most common substrates have been subject of accelerated weathering. Samples were watered by distilled water, left to soak in, then froze, thaw and dried. This cycle was repeated twenty times. Before the experiment and after experiment pH, conductivity, organic matter loss on ignition, grain size and water absorption were measured. Before and after the experiment were tested for toxicity to plants using yellow mustard seeds (Sinapis alba). Accelerated weathering showed the possible development of weathering spoil substrates over time namely shift of pH towards neutral value, and increase in water holding capacity, despite of this no changes in plant toxicity in toxic substrates were found.
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The effect of toxicity and othet parameters of substrate on plant growth in post mining sites.
Zadinová, Radka ; Frouz, Jan (advisor) ; Kočí, Vladimír (referee)
Surface mining of coal in the country leaves a lot of damage. This type of mining is characterized by the creation of large external dumps. The dumps often contain substrates with toxic substances or substances which become toxic after reaction with air. These substrates are toxic to plants and edaphon. This work is intended to show what properties of substrates are important for phytotoxicity. The location of research was selected including 3 different kinds of substrates, terrain unevenness and a lagoon in the middle. Coordinate grid has been created on the selected location. At each point a test was conducted with plant Sinapis alba and biomass was extracted from a naturally growing vegetation. Further, at the points substrates were collected and values of pH, conductivity and arsenic were measured. Test with plant Sinapis alba was repeated in the laboratory conditions as well. On site GPS coordinates were measured for the mathematical model created in ArcGIS. Experiment has shown the importance of the type of substrate and the geologic description, and then the association between dejection, value of pH and conductivity. Conductivity and pH levels also correlate with the germination Sinapis alba and biomass of naturally growing vegetation.
|
|
|
The effect of artificial weathering on overburden properties
Zadinová, Radka ; Bartuška, Martin (referee) ; Frouz, Jan (advisor)
Surface mining of coal cause massive changes of landscape. Dumps, heaps and tailings created vast areas in post mining landscape. Soil formation is influenced by the weathering process. Sample-the most common substrates have been subject of accelerated weathering. Samples were watered by distilled water, left to soak in, then froze, thaw and dried. This cycle was repeated twenty times. Before the experiment and after experiment pH, conductivity, organic matter loss on ignition, grain size and water absorption were measured. Before and after the experiment were tested for toxicity to plants using yellow mustard seeds (Sinapis alba). Accelerated weathering showed the possible development of weathering spoil substrates over time namely shift of pH towards neutral value, and increase in water holding capacity, despite of this no changes in plant toxicity in toxic substrates were found.
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UTILIZATION OF ALTERNATIVE TESTS OF BIOTOXICITY FOR DETERMINATION INADVISABILITY OF EXISTING AND/OR POTENTIONAL POLUTANTS
Vašulková, Lucie ; Zlámalová Gargošová, Helena (referee) ; Bednařík, Karel (advisor)
This thesis is focused on attestation of ecotoxicity of octachlorstyrene (OCS), persistent organic pollutant (POP), by biological tests of toxicity. The toxicity of OCS was determined by using two kinds of alternative tests applied on fresh water organisms. First of them, Thamnotoxkit, is based on observation of mobility, immobilization or even killing of crustacean Thamnocephalus platyurus. The second one, Daphnotoxkit, applies crustacean Daphnia magna. For purposes of comparison, a standard test of toxicity – so called test of root growth inhibition of Sinapis alba and two fytotests – test of growth inhibition of Lemna minor and test of root growth inhibition of Allium cepa were performed as well.
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