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Optimalization of techniques of microrheology for characterization of biopolymer hydrogels
Dušenková, Alica ; Smilek, Jiří (referee) ; Sedláček, Petr (advisor)
The main aim of the bachelor thesis is optimalization of microrheological techniques for characterization of biopolymer hydrogels. Hydrogels, based on thermoreversible biopolymer agarose, were selected for these experiments. The influence of incorporated poly(sodium 4-styrenesulfonate) on structure and viscoelastic properties of agarose hydrogels was investigated through diffusion coefficient and MSD curves. Microrheological properties were studied by fluorescence correlation spectroscopy, by using fluorescent beads, which were embedded in hydrogel systems.
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Diffusion characterization of hydrogels at microscopic level
Uher, Tadeáš ; Smilková, Marcela (referee) ; Sedláček, Petr (advisor)
This Bachelor’s thesis deals with study of diffusion in gels on microscopic level. The method of fluorescence correlation spectroscopy is used for the study of diffusion and this method is based on monitoring the diffusion coefficients in a confocal volume of studied substance. As fluorescence probe is used organic pigment called Rhodamin 6G and diffusion experiments are realized in hydrogel matrix, whose medium forms thermoreversible linear polycarbohydrate – agarose, with addition of reactive component – biopolymer called sodium polystyrenesulphate in certain concentrations. The purpose of the thesis is to research the impacts of interaction between diffusing pigment and specific type of hydrogel. The change of final diffusion coefficient is observed. It is assumed, that sodium polystyrenesulphate influences the diffusion of fluorophore (Rhodamin 6G) in agarose hydrogel.
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Diffusion characterization of hydrogels at microscopic level
Uher, Tadeáš ; Smilková, Marcela (referee) ; Sedláček, Petr (advisor)
This Bachelor’s thesis deals with study of diffusion in gels on microscopic level. The method of fluorescence correlation spectroscopy is used for the study of diffusion and this method is based on monitoring the diffusion coefficients in a confocal volume of studied substance. As fluorescence probe is used organic pigment called Rhodamin 6G and diffusion experiments are realized in hydrogel matrix, whose medium forms thermoreversible linear polycarbohydrate – agarose, with addition of reactive component – biopolymer called sodium polystyrenesulphate in certain concentrations. The purpose of the thesis is to research the impacts of interaction between diffusing pigment and specific type of hydrogel. The change of final diffusion coefficient is observed. It is assumed, that sodium polystyrenesulphate influences the diffusion of fluorophore (Rhodamin 6G) in agarose hydrogel.
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Optimalization of techniques of microrheology for characterization of biopolymer hydrogels
Dušenková, Alica ; Smilek, Jiří (referee) ; Sedláček, Petr (advisor)
The main aim of the bachelor thesis is optimalization of microrheological techniques for characterization of biopolymer hydrogels. Hydrogels, based on thermoreversible biopolymer agarose, were selected for these experiments. The influence of incorporated poly(sodium 4-styrenesulfonate) on structure and viscoelastic properties of agarose hydrogels was investigated through diffusion coefficient and MSD curves. Microrheological properties were studied by fluorescence correlation spectroscopy, by using fluorescent beads, which were embedded in hydrogel systems.
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