|
|
Characterisation and regulation of muscarinic and adrenergic receptors Subtitle: The effect of stress on muscarinic and adrenergic receptors in the lung and in the heart
Nováková, Martina ; Mysliveček, Jaromír (advisor) ; Hynie, Sixtus (referee) ; Mravec, Boris (referee)
The aim of this thesis was to clarify the influence of the stress on the adrenergic and muscarinic receptors in the heart and in the lungs. Research was perform on rat hearts and lungs and on the hearts and lungs of the CRH KO mice. First, we assessed mRNA levels of all α- and β-adrenergic receptor and muscarinic receptor subtypes. Subsequently, we performed the radioligand-binding studies to determine densities of these receptors. We identified all three α1-adrenergic receptor subtypes in the rat lungs. In the lungs of WT mice, we found that the amount of α1-adrenergic and muscarinic receptors was sex-dependent. Densities of the former were higher in females and those of the latter were higher in males. There was no difference between males and females in β-adrenergic receptor density. As for CRH KO mice, the basal densities of studied receptors were lower than in CRH WT mice (except β1-adrenergic receptors in females). The main purpose of the thesis was to detect the immobilization-induced changes in the studied receptors in the kontrol (WT) and CRH KO mice. Short-term and long-term immobilization caused decrease in all α1-adrenergic receptor subtypes in females, whereas only α1A-adrenergic receptors decreased in males. The amount of β1-adrenergic receptors decreased in males and remained without...
|
|
|
Mortality of females from selected malignant neoplasms in the context of preventive programs in the Czech and Slovak Republic since 1994
Antošová, Marie ; Hulíková Tesárková, Klára (advisor) ; Fialová, Ludmila (referee)
Mortality of females from selected malignant neoplasms in the context of preventive programs in the Czech and Slovak Republic since 1994 Abstract The main aim of the thesis is to compare the differences in mortality due to female malignant neoplasms (neoplasm of the breast, cervix and colon) in the Czech Republic and Slovak Republic during 1994-2015. Changes in mortality levels have been associated with the implementation of relevant preventive programs. The development of mortality is analyzed according to age only for female population and causes of death using the standardized mortality rate of selected causes of death and age specific mortality rate and using the decomposition of life expectancy at birth between the years 1994-2001 and 2009-2015 in terms of age groups and causes of death. Results of the analytical part of the thesis show that mortality decreased during 1994-2015 but the differences among mortality in both selected countries occurred. Mortality in both the analyzed countries differed above all for malignant neoplasm of the breast. Higher efficiency of established preventive programs has been demonstrated in the Czech Republic than in the Slovak Republic. Keywords: Czech Republic, Slovak Republic, mortality by causes of death, females, prevention programs, malignant neoplasms, decomposition
|
|
|
Characterisation and regulation of muscarinic and adrenergic receptors Subtitle: The effect of stress on muscarinic and adrenergic receptors in the lung and in the heart
Nováková, Martina ; Mysliveček, Jaromír (advisor) ; Hynie, Sixtus (referee) ; Mravec, Boris (referee)
The aim of this thesis was to clarify the influence of the stress on the adrenergic and muscarinic receptors in the heart and in the lungs. Research was perform on rat hearts and lungs and on the hearts and lungs of the CRH KO mice. First, we assessed mRNA levels of all α- and β-adrenergic receptor and muscarinic receptor subtypes. Subsequently, we performed the radioligand-binding studies to determine densities of these receptors. We identified all three α1-adrenergic receptor subtypes in the rat lungs. In the lungs of WT mice, we found that the amount of α1-adrenergic and muscarinic receptors was sex-dependent. Densities of the former were higher in females and those of the latter were higher in males. There was no difference between males and females in β-adrenergic receptor density. As for CRH KO mice, the basal densities of studied receptors were lower than in CRH WT mice (except β1-adrenergic receptors in females). The main purpose of the thesis was to detect the immobilization-induced changes in the studied receptors in the kontrol (WT) and CRH KO mice. Short-term and long-term immobilization caused decrease in all α1-adrenergic receptor subtypes in females, whereas only α1A-adrenergic receptors decreased in males. The amount of β1-adrenergic receptors decreased in males and remained without...
|
|
|
Induction and optimalization out-of-season spawning of spawners pikeperch (Sander lucioperca)
VLČEK, Jakub
In total, 21 pairs of pikeperch brood stock were divided into three groups and used during two out-of-season and one seasonal semi-artificial spawning. The only differences among these three groups were the length of cold water period (CWP) and spawning date. Group of earlier spawning (A) CWP = 122 days, Group of normal spawning (B) CWP = 149 days, Group of late spawning (C) CWP = 223 days). Dates of spawnings A 11.3., B 26.4., C 13.6). There were observed and assessed the main production parameters such us latency, successful spawning, fertilisation and hatching rate etc. in each group and spermatozoa quality (sampled one day after the observed spawning). In Group A and B, 100 % of fish successfully spawned. Fertilisation rate was 59.4 % (Group A) and 80.3 % (Group B). Much worse results were obtained in Group C. Only 42.9 % of fish spawned and the fertilisation rate was zero. There was no difference among the three groups in spermatozoa quality parameters. It means that the poor fertilisation rate in Group C was caused by low egg quality. According to our results we can say that it is possible to successfully spawn pikeperch earlier (before the main spawning season) with comparable result to the natural spawning season. However, it is necessary to find some better way for female stimulation to provide higher egg quality and subsequently higher fertilisation rate.
|
guest ::
