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Production of extracellular hydrolases by specific mould species
Pokrývková, Zuzana ; Skoumalová, Petra (referee) ; Márová, Ivana (advisor)
This bachelor's thesis is focused on studying the possibilities of producing extracellular hydrolytic enzymes by the fungus Penicillium lilacinum on two substrates. The theoretical part is focused on the characterization of selected hydrolytic enzymes, their characteristics, the possibilities of production and their applications. In the experimental part the production of hydrolytic enzymes by the fungus Penicillium lilacinum was performed in mineral medium and in a medium wherein as substrate yeast strains Sporobolomyces roseus and Rhodotorula glutinis were used. During cultivation process, production of cellulases, amylases, xylanases, lipases, proteases, and mannases was monitored. Samples were taken on 3rd, 5th, 8th, 10th and 12th day of cultivation. Production varies depending on time and substrate type. Amylase, cellulose and xylanase activity was measured in two steps - at the beginning of culture (3 to 5 day) and at the end of cultivation. The rest of enzymes were detected mainly in the 8th to 10th day of culture. The best three samples with the highest value of enzyme activities were further used for lyofilization and purification by ultrafiltration. Further, enzymes were tested for disruption of yeast cell wall. In the conclusion, the yeast protoplasts of yeast strains Rhodotorula glutinis a Sporobolomyces roseus were prepared successfully.
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Characetrization of selected microbial enzymes
Bradáčová, Kristína ; Hlaváček, Viliam (referee) ; Márová, Ivana (advisor)
This bachelor´s thesis is focused on controlled production and identification of extracellular microbial hydrolytic enzymes by fungi. Theoretical part deals with characterization of selected hydrolytic enzymes, their properties, possibility of production and application. In experimental part the production of enzymes by fungal strains Phanerochaete chrysosporium, Aureobasidium pullulans and Aspergillus oryzae was performed. Cultivation was conducted in submersed mode in mineral medium and in media with waste co-substrates such as wheat bran, sawdust, rapeseed cake (lipids content 2,55 %) and rapeseed cake rich in lipids (9 %). The activity of cellulases, xylanases, amylases, ligninperoxidase, manganese-dependent peroxidase and laccase was monitored during cultivation process and regularly on 3rd, 7th, 10th and 15th day of cultivation. Production of enzymes depended on time and the subsrate type. Cellulases and xylanases were produced mainly on 3rd and 7th day of cultivation, amylases on 3rd and 15th day and lignolytic enzymes on 7th and 15th day. Samples were further separated and analyzed by ultrafiltration, gel filtration and PAGE-SDS electroforesis.
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Characetrization of selected microbial enzymes
Bradáčová, Kristína ; Hlaváček, Viliam (referee) ; Márová, Ivana (advisor)
This bachelor´s thesis is focused on controlled production and identification of extracellular microbial hydrolytic enzymes by fungi. Theoretical part deals with characterization of selected hydrolytic enzymes, their properties, possibility of production and application. In experimental part the production of enzymes by fungal strains Phanerochaete chrysosporium, Aureobasidium pullulans and Aspergillus oryzae was performed. Cultivation was conducted in submersed mode in mineral medium and in media with waste co-substrates such as wheat bran, sawdust, rapeseed cake (lipids content 2,55 %) and rapeseed cake rich in lipids (9 %). The activity of cellulases, xylanases, amylases, ligninperoxidase, manganese-dependent peroxidase and laccase was monitored during cultivation process and regularly on 3rd, 7th, 10th and 15th day of cultivation. Production of enzymes depended on time and the subsrate type. Cellulases and xylanases were produced mainly on 3rd and 7th day of cultivation, amylases on 3rd and 15th day and lignolytic enzymes on 7th and 15th day. Samples were further separated and analyzed by ultrafiltration, gel filtration and PAGE-SDS electroforesis.
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Production of extracellular hydrolases by specific mould species
Pokrývková, Zuzana ; Skoumalová, Petra (referee) ; Márová, Ivana (advisor)
This bachelor's thesis is focused on studying the possibilities of producing extracellular hydrolytic enzymes by the fungus Penicillium lilacinum on two substrates. The theoretical part is focused on the characterization of selected hydrolytic enzymes, their characteristics, the possibilities of production and their applications. In the experimental part the production of hydrolytic enzymes by the fungus Penicillium lilacinum was performed in mineral medium and in a medium wherein as substrate yeast strains Sporobolomyces roseus and Rhodotorula glutinis were used. During cultivation process, production of cellulases, amylases, xylanases, lipases, proteases, and mannases was monitored. Samples were taken on 3rd, 5th, 8th, 10th and 12th day of cultivation. Production varies depending on time and substrate type. Amylase, cellulose and xylanase activity was measured in two steps - at the beginning of culture (3 to 5 day) and at the end of cultivation. The rest of enzymes were detected mainly in the 8th to 10th day of culture. The best three samples with the highest value of enzyme activities were further used for lyofilization and purification by ultrafiltration. Further, enzymes were tested for disruption of yeast cell wall. In the conclusion, the yeast protoplasts of yeast strains Rhodotorula glutinis a Sporobolomyces roseus were prepared successfully.
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