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Kinetic study of excited state proton transfer in polymer-surfactant system
Bačová, Jana ; Vala, Martin (referee) ; Mravec, Filip (advisor)
In this bachelor thesis were studied interactions between hyaluronic acid and cationic septonex by steady-state and time-resolved fluorescence with fluorescence probes pyrene and 1-naphthol. At first was determined critical micelle concentration of septonex. In the case of both fluorescence probes was studied area below and above critical micelle concentration. It was found, that 1-naphthol undergo very fast deprotonation in aqueous solution, while in the present of micelles the rate of deprotonation is c significantly retarded. In the system of hyaluronic acid – septonex it was observed, that formation of aggregates occurs at lower concentrations than in the system without hyaluronic acid. Molecules of probe are probably distributed between hyaluronic acid – septonex aggregates and free micelles above critical micelle concentration.
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Time-Resolved Fluorescence in Research of Hyaluronan-Colloidal Systems Interactions
Mondek, Jakub ; Táborský, Petr (referee) ; Peter, Kapusta (referee) ; Pekař, Miloslav (advisor)
The aim of the doctoral thesis was to study advanced fluorescence techniques and its use in colloids or hyaluronan-surfactant systems and hydrogels based on hyaluronan, respectively. Steady-state and time-resolved fluorescence were used to study excited state proton transfer fluroescen probes in hyaluronan-surfactant systems to asses the influence of hyaluronan hydration to its interactions with oppositely charged surfactants. Firstly, different excited state proton transfer fluorescence probes were discussed to choose the most suitable candidate for next research. The influence of hyaluronan on inner environment of micells was determined based on the sensitivity of excited state proton transfer of chosen fluorescence probe 1-naphtol and, based on above mentioned experiments, the structure of hyaluronan hydration shell was discussed. The next part of doctoral thesis was focused on fluorescence lifetime correlation spectroscopy and on the development of method of nanorheology. Measured correlation functions were transformed to mean square displacement with developed MATLAB script. Firstly, the fluorescence method was compared with well described methods such as videomicrorheology and dynamic light scattering to asses the reliability of fluorescence correlation spectroscopy in microrheology. Secondly, nanorheology method was developed and its use in passive nanorheology of hyaluronan hydrogels was discussed. Based on mentioned experiments, the fluorescence correlation spectroscopy microrheology and nanorheology methods were optimized to use the methods in hydrogel research.
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Time-Resolved Fluorescence in Research of Hyaluronan-Colloidal Systems Interactions
Mondek, Jakub ; Táborský, Petr (referee) ; Peter, Kapusta (referee) ; Pekař, Miloslav (advisor)
The aim of the doctoral thesis was to study advanced fluorescence techniques and its use in colloids or hyaluronan-surfactant systems and hydrogels based on hyaluronan, respectively. Steady-state and time-resolved fluorescence were used to study excited state proton transfer fluroescen probes in hyaluronan-surfactant systems to asses the influence of hyaluronan hydration to its interactions with oppositely charged surfactants. Firstly, different excited state proton transfer fluorescence probes were discussed to choose the most suitable candidate for next research. The influence of hyaluronan on inner environment of micells was determined based on the sensitivity of excited state proton transfer of chosen fluorescence probe 1-naphtol and, based on above mentioned experiments, the structure of hyaluronan hydration shell was discussed. The next part of doctoral thesis was focused on fluorescence lifetime correlation spectroscopy and on the development of method of nanorheology. Measured correlation functions were transformed to mean square displacement with developed MATLAB script. Firstly, the fluorescence method was compared with well described methods such as videomicrorheology and dynamic light scattering to asses the reliability of fluorescence correlation spectroscopy in microrheology. Secondly, nanorheology method was developed and its use in passive nanorheology of hyaluronan hydrogels was discussed. Based on mentioned experiments, the fluorescence correlation spectroscopy microrheology and nanorheology methods were optimized to use the methods in hydrogel research.
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Kinetic study of excited state proton transfer in polymer-surfactant system
Bačová, Jana ; Vala, Martin (referee) ; Mravec, Filip (advisor)
In this bachelor thesis were studied interactions between hyaluronic acid and cationic septonex by steady-state and time-resolved fluorescence with fluorescence probes pyrene and 1-naphthol. At first was determined critical micelle concentration of septonex. In the case of both fluorescence probes was studied area below and above critical micelle concentration. It was found, that 1-naphthol undergo very fast deprotonation in aqueous solution, while in the present of micelles the rate of deprotonation is c significantly retarded. In the system of hyaluronic acid – septonex it was observed, that formation of aggregates occurs at lower concentrations than in the system without hyaluronic acid. Molecules of probe are probably distributed between hyaluronic acid – septonex aggregates and free micelles above critical micelle concentration.
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