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Electrochemical characterization of bioelectronic systems
Runštuková, Nikola ; Salyk, Ota (referee) ; Ehlich, Jiří (advisor)
The aim of this thesis was electrochemical characterization of 24 microbial fuel cells platform for high-throughput screening of electrogenic microorganisms and comparison of measured values across all the fuel cells. In the theoretical part, there is a description of electrogenic bacteria, which are essential for current genereation in microbial fuel cells, description of microbial fuel cells and methods used for their characterization. In experimental part, there is a description of assembly process of the platform and measurement process. The result of this thesis is characterization of the platform before and after inoculation using open-circuit potential measurement, continuous current measurement, cyclic voltametry, linear sweep voltametry and electrochemical impedance spectroscopy. Thanks to this methods the values of open-circuit potencial, maximum power and internal resistance were obtained and their distribution was compared. These performed experiments verified the correct function of presented fuel cell platform.
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Electrochemical characterization of bioelectronic systems
Runštuková, Nikola ; Salyk, Ota (referee) ; Ehlich, Jiří (advisor)
The aim of this thesis was electrochemical characterization of 24 microbial fuel cells platform for high-throughput screening of electrogenic microorganisms and comparison of measured values across all the fuel cells. In the theoretical part, there is a description of electrogenic bacteria, which are essential for current genereation in microbial fuel cells, description of microbial fuel cells and methods used for their characterization. In experimental part, there is a description of assembly process of the platform and measurement process. The result of this thesis is characterization of the platform before and after inoculation using open-circuit potential measurement, continuous current measurement, cyclic voltametry, linear sweep voltametry and electrochemical impedance spectroscopy. Thanks to this methods the values of open-circuit potencial, maximum power and internal resistance were obtained and their distribution was compared. These performed experiments verified the correct function of presented fuel cell platform.
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Testing of new electrode arrangements for monitoring of electrochemically oxidisable biologically active organic compounds
Libánský, Milan
Submitted Ph.D. Thesis is focused on the electrochemical characterization and testing of recently developed working electrodes made from pure gold or graphitic carbon particles and electrochemical arrangements. These electrodes are suitable for large screening measurements of various organic compounds. The development of new sensitive voltammetric methods for determination of oxidisable biologically active organic compounds is another aim of this work. To verify its applicability, the array of carbon composite film electrodes integrated in measuring cell system was selected for the development of voltammetric methods for determination of homovanillic acid, vanillylmandelic acid, and indoxyl sulphate. These analytes, which belong to the group of biomarkers of human diseases, were selected for increasing interest in their determination in medical laboratories. Moreover, determination of indoxyl sulphate was coupled to its solid phase extraction from human urine prior to voltammetric determination. Obtained results were compared with measurements of standards with well-established carbon paste electrode. Sputtered (physical vapour deposition method) gold nanostructured film electrodes on treated PTFE substrates and gold nanostructured film electrodes modified with various functional groups on the...
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Testing of new electrode arrangements for monitoring of electrochemically oxidisable biologically active organic compounds
Libánský, Milan
Submitted Ph.D. Thesis is focused on the electrochemical characterization and testing of recently developed working electrodes made from pure gold or graphitic carbon particles and electrochemical arrangements. These electrodes are suitable for large screening measurements of various organic compounds. The development of new sensitive voltammetric methods for determination of oxidisable biologically active organic compounds is another aim of this work. To verify its applicability, the array of carbon composite film electrodes integrated in measuring cell system was selected for the development of voltammetric methods for determination of homovanillic acid, vanillylmandelic acid, and indoxyl sulphate. These analytes, which belong to the group of biomarkers of human diseases, were selected for increasing interest in their determination in medical laboratories. Moreover, determination of indoxyl sulphate was coupled to its solid phase extraction from human urine prior to voltammetric determination. Obtained results were compared with measurements of standards with well-established carbon paste electrode. Sputtered (physical vapour deposition method) gold nanostructured film electrodes on treated PTFE substrates and gold nanostructured film electrodes modified with various functional groups on the...
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Testing of new electrode arrangements for monitoring of electrochemically oxidisable biologically active organic compounds
Libánský, Milan ; Zima, Jiří (advisor) ; Navrátil, Tomáš (referee) ; Šelešovská, Renáta (referee)
Submitted Ph.D. Thesis is focused on the electrochemical characterization and testing of recently developed working electrodes made from pure gold or graphitic carbon particles and electrochemical arrangements. These electrodes are suitable for large screening measurements of various organic compounds. The development of new sensitive voltammetric methods for determination of oxidisable biologically active organic compounds is another aim of this work. To verify its applicability, the array of carbon composite film electrodes integrated in measuring cell system was selected for the development of voltammetric methods for determination of homovanillic acid, vanillylmandelic acid, and indoxyl sulphate. These analytes, which belong to the group of biomarkers of human diseases, were selected for increasing interest in their determination in medical laboratories. Moreover, determination of indoxyl sulphate was coupled to its solid phase extraction from human urine prior to voltammetric determination. Obtained results were compared with measurements of standards with well-established carbon paste electrode. Sputtered (physical vapour deposition method) gold nanostructured film electrodes on treated PTFE substrates and gold nanostructured film electrodes modified with various functional groups on the...
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