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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Synthesis and study of properties of anchors with multiple positive permanent charges and allyl group
 Kozel, Ondřej ; Jindřich, Jindřich (advisor) ; Kamlar, Martin (referee)
Solid surface modification is a widely researched topic where there is mostly used covalent bonding. Such modifications are advantageous due to the strength of the newly formed bond but are inconvenient for their financial and material expensiveness. There were also investigated the possibilities of ionic modifications, which are using electrostatic interactions, but which are not widely used-the reason being the weak ionic bond which leads to washing out of the modifier by polar solvents. Nevertheless, new substances were designed which should be more resistant against this issue due to their structure. These substances, containing the propargyl group, were tested in solid surfaces modification. This bachelor work demonstrates the usability of procedures designed for the preparation of such substances with the allylic group. Keywords: permanent positive charges; click reaction; electrostatic binding; ionic modification; quaternary ammonium salts; solid surface modification
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New method of peptide separation using electrostatic binding
 Palágyi, Attila ; Jindřich, Jindřich (advisor) ; Smrček, Stanislav (referee)
The diploma thesis deals with the development of a new method of synthetic peptide separation using electrostatic binding. It deals with the development of a multi-charged anchored linker attachable to a synthetic peptide after its synthesis on solid phase. Subsequently, it investigates the separation using electrostatic binding; however, after peptide cleavage from the anchored linker and Boc deprotection with TFA, the peptide formed so strong electrostatic interactions with the cation exchanger that we were unable to recover it. The thesis is finished with discussion and method improvement proposal. Key words: peptide separation, solid-phase peptide synthesis, SPPS, electrostatic binding
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