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Influence of electrical pulses on human blood phagocytes
Chorvátová, Michaela ; Číž,, Milan (referee) ; Kubala,, Lukáš (advisor)
The phagocytic cells circulating in the bloodstream play a key role in both the defense of the body and the pathology of inflammatory diseases. Thus, targeting their functions has potential to modulate an immune response, especially during the inflammatory phase. This master's thesis was focused on the influence of electric pulses on the most abundant phagocyte population in human peripheral blood, namely neutrophils. The theoretical part describes the role of neutrophils in the development of the immune response and the effects of the electric field on various cells. Consequent part of the thesis was the optimization of the electrical stimulation of neutrophils using a unique platform with a network of gold electrodes. In stimulated cells by electrical pulses, activation of selected signaling pathways, degranulation, ROS production, citrullination of histone H3 and expression of surface markers were monitored. Overall, electrical stimulation was observed to induce neutrophil activation but only electrical pulses of size 1 V were found to be statistically significant in the case of ROS production and 10 mV and 100 mV electrical pulses in the case of metalloproteinase MMP8 degranulation. The absence of significant effects in the most observed parameters was probably due to unwanted activation of neutrophils in control samples.
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Tensiomyography – measurement uncertainty analysis
Sikorová, Pavlína ; Mornstein, Vojtěch (referee) ; Vlk, Daniel (advisor)
This bachelor’s thesis deals with the analysis of measurement uncertainty during the tensiomyographic measuring. Seven factors have been suggested (i.e. uncertainties), that may cause an error in measurement. Their influence on the measured parameters was tested in a series of experiments on m. biceps femoris and m. biceps brachii of two subjects. The results of these tests were then compared with the referential values from the physiotherapist and the device manufacturer. Based on this comparison, the extent of uncertainty of diagnostically essential parameters Dm and Dc has been designated. Dm is heavily influenced by six parameters out of seven, whereas Tc is influenced only by one.
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Tensiomyography – measurement uncertainty analysis
Sikorová, Pavlína ; Mornstein, Vojtěch (referee) ; Vlk, Daniel (advisor)
This bachelor’s thesis deals with the analysis of measurement uncertainty during the tensiomyographic measuring. Seven factors have been suggested (i.e. uncertainties), that may cause an error in measurement. Their influence on the measured parameters was tested in a series of experiments on m. biceps femoris and m. biceps brachii of two subjects. The results of these tests were then compared with the referential values from the physiotherapist and the device manufacturer. Based on this comparison, the extent of uncertainty of diagnostically essential parameters Dm and Dc has been designated. Dm is heavily influenced by six parameters out of seven, whereas Tc is influenced only by one.
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Influence of electrical pulses on human blood phagocytes
Chorvátová, Michaela ; Číž,, Milan (referee) ; Kubala,, Lukáš (advisor)
The phagocytic cells circulating in the bloodstream play a key role in both the defense of the body and the pathology of inflammatory diseases. Thus, targeting their functions has potential to modulate an immune response, especially during the inflammatory phase. This master's thesis was focused on the influence of electric pulses on the most abundant phagocyte population in human peripheral blood, namely neutrophils. The theoretical part describes the role of neutrophils in the development of the immune response and the effects of the electric field on various cells. Consequent part of the thesis was the optimization of the electrical stimulation of neutrophils using a unique platform with a network of gold electrodes. In stimulated cells by electrical pulses, activation of selected signaling pathways, degranulation, ROS production, citrullination of histone H3 and expression of surface markers were monitored. Overall, electrical stimulation was observed to induce neutrophil activation but only electrical pulses of size 1 V were found to be statistically significant in the case of ROS production and 10 mV and 100 mV electrical pulses in the case of metalloproteinase MMP8 degranulation. The absence of significant effects in the most observed parameters was probably due to unwanted activation of neutrophils in control samples.
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