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Kvalitativní a kvantitativní ukazatele ejakulátu kohoutů výchozích snáškových linií kura domácího (gallus domesticus)
Bestová, Barbora
Bachelor's thesis describes the qualitative and quantitative indicators of roosters ejaculate. Literary review deals with the reproductive system of domestic fowl, the composition of the ejaculate, sperm morphology and spermatogenesis. It also deals with macroscopic and microscopic evaluation of semen. It altso describes the morphological defects in sperm. The methodology to evaluate the qualitative and quantitative indicators of ejaculate of two laying breeds - Rhode Island White and Brindle Plymouth Rocsk. Both breeds have fewer than 20% of morphologically abnormal sperm. Most of the changes were represented at the connecting piece, the head and a tail.
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Hodnocení kvalitativních a kvantitativních ukazatelů ejakulátu u hřebců
Hájková, Ivana
Evaluation of semen quality is an important element in the evaluation of stallion fertility. Quality ejaculate produced in sufficient quantities is a prerequisite for successful insemination in horse breeding. To assess the quality of ejaculate is used by many laboratory evaluation methods. Selected methods are also engaged in this thesis. The basic parameters considerating in the ejaculate in this thesis are the evaluation of activity, sperm concentration and morphological sperm analysis. For using ejaculate in producing insemination doses need to be assessed volume of ejaculate too. Minimum volume of the ejaculate of stallions should be 20 ml. The activity of fresh semen should be at least 60 %. Normal sperm concentration value of stallions ejaculate is considered at least 100 × 106 in 1 ml of semen. The minimum amount of morphologically normal stallions sperm is required above the threshold value of 60 %. In this thesis we studied a group of stallions which were stabled in Zemský hřebčinec Tlumačov, s.p. Stallions were included in breeding season for 2015. Evaluation of quantitative and qualitative parameters were performed od 47 samples of fresh semen, which were collected from 12 stallions during the breeding season. Semen collecting was from April to July 2015. Immediately after each semen collecting was measured volume of ejaculate and sperm activity was recorded. To further evaluation were counting sperm concentration and morphology of sperm evaluation. For mophological analysis were preparations stained by two methods: B. T. Farelly staining and method Byghošť. The analysis was determined by the percentage of morphological sperm defects, which were divided into the following categories: acrosome defects, sperm head defects, sperm mid-piece defects, sperm tail defects, abnormal forms of sperm and immature sperm. The research also used a theoretical comparison of the two methods of stainings due to occurence of morphological defects and estimate the correlation relationships between qualitative and quantitative parameters of the ejaculated os stallions.
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Výživa plemenných býků
KOUBA, Jindřich
The aim of this thesis was the study of available publications covering the topic of breeding bulls nutrition, the analysis of the feed dose and the evaluation of the semen quantity and quality after addition of extruded flaxseed (EL) to the feed dose. 200 semen samples from 8 breeding bulls of the Czech Fleckvieh were proposed as the subject of observation. The influence of EL to the sperm motility and concentration and to the amount of semen was observed and evaluated. The positive effect was found out in all of the observed characteristics. Experimental group showed increase in the amount of semen in comparison to the control group, and the statistical analysis displayed a significant difference in the effect of the time factor (effect of the addition of EL) on the amount of semen within the groups. The sperm concentration in 1 mm3 of semen of the experimental group increased compared to the control group, but the difference was not statistically significant. The experimental group showed an increase in sperm activity and the time factor was statistically significant. From the results of this thesis follows that extruded flaxseed can be recommended as the suitable feed supplement for breeding bulls.
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Effect of equilibration and freezing parameters on bull sperm motility after thawing
Beránková, Monika ; Stádník, Luděk (advisor) ; Zuzana, Zuzana (referee)
The hypothesis assume the existence differences in bulls spermatozoa viability after thawing, characterized by higher values of motility and higher proportion of live spermatozoa, in case of prolonged equilibration and lower temperature freezing gradient. The aim of this thesis was to find the influence of different equilibration and freezing curve conditions to on spermatozoa viability after thawing. The theoretical part of the thesis summarizes available information about the bulls semen parameters, methods for its evaluation and especially the processing and long-term preservation.
Within the case study part of the thesis, during the years 2013-2015 the semen of the pre-selected group of 5 breeding bulls was being collected at the Sires insemination center. The group was uniform as to age, breed and housing and treatment conditions. After initial assessment the collected semen was divided into parts, which were then processed in different wals. A part of straws was processed by standard protocol, the other part was processed using different length of equilibration and freezing curve.
First part of the straws was equilibrated in a standard protocol for 120 minutes; subsequently half of the equilibrated straws was standardly frozen by the Direct Freezing method based on 3-phase freezing curve while the other half was frozen using the different 2-phase freezing curve. Second part of the straws was initially equilibrated for 240 minutes with the subsequent freezing, similarly as in the previous case, used 3-phase and 2-phase freezing curve. Frozen straws were then stored in liquid nitrogen container at -196 °C.
Spermatozoa motility was evaluated using CASA. The semen was further evaluated on the proportion of live and dead spermatozoa, using coloration of Eosin and Nigrosine, and on the sensitivity of the spermatozoa membranes, using HOS test, which detects the reaction of spermatozoa tail membrane to hypoosmotic conditions.
The obtained results indicated higher progressive spermatozoa motility (+5.57 %) and higher percentage of live spermatozoa (+4.47 %) following the application of the prolonged equilibration (240 min). When applying the length of equilibration 240 minutes, the values of total spermatozoa motility and HOS test were higher as well, though the differences were not statistically significant. When evaluate the effect of using different freezing curves on the parameters of the spermatozoa after thawing, higher values (average + 1.6 %) at almost all of these parameters using 3-phase freezing curve were found, the diferrences were not statistically significant.
Statistically significant differences among the bulls (P < 0.01) were found in total as well as progressive spermatozoa motility, in the proportion of living spermatozoa and in value of the HOS test - the individuality among the bulls is therefore evident. While evaluating the characteristics of spermatozoa movement by CASA, statistically significant differences among the bulls were confirmed at VCL, VSL, VAP, LIN, STR, WOB (P < 0.01), also between the diferrent length of equilibration (P < 0.01), but were not statistically significant when using diferrent freezing curves.
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Zhodnocení kvality ejakulátu vybraných plemen psů
Hronková, Petra
The theoretical part of the thesis on "Evaluation of semen quality selected breeds of dogs", provides a brief summary of available information obtained from literature dealing with dogs reproduction, anatomy male genital tract of the dog and basic laboratory techniques of animal semen analyzes. Practical work was focused on evaluating quantitative and qualitative parameters of ejaculate different breeds of dogs from the kennel Bohemia Alké. Was investigated primarily affect the order of collection, ages, body frame, time of day and length of the sampling collection itself on ejaculate volume, activity concentration, total sperm count and percentage of occurrence patomorfologických defects. Subscriptions ejaculate, macroscopic and microscopic evaluation was conducted in field conditions in the home where the dogs lived. To evaluate the percentage of damaged sperm pathomorphological were made morphological coatings, which were transported to the laboratory department reproduction of farm animals and the Institute of Animal Breeding, Faculty of Agronomy, Mendel University in Brno.
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Vliv stájového mikroklimatu na vybrané kvalitativní a kvantitativní parametry ejakulátů plemenných býků
Škarecká, Radka
The aim of this diploma thesis The effect of stable microclimate on qualitative and quantitative parameters of ejaculates of breeding bulls, where the effect of stable envi-ronment and age on the volume, concentration, activity and changes in morphology of sperm in ejaculates of bulls was evaluated. In the period from 1st March to 31st August 2014, there were done 154 collections of semen from nine bulls of Czech Pied cattle lairaged at the insemination center of bulls operated by a breeder's cooperative Impuls in Bohdalec. Immediately after the collections macroscopic and microscopic examinations have been performed for all samples, which included determining the volume, con-centration and activity of sperm. After evaluation of morphological parameters of bovi-ne sperm the percentage of morphologically normal sperm and pathomorphological da-maged sperm were evaluated. There were observed changes on the head, acrosome, connecting part and flagellum. Our results show that the biggest effect on the qualitative and quantitative parameters of semen for breeding bulls has age, the temperature along with moisture, where the greatest differences in results were found for the extreme va-lues. A value of temperature - humidity index (THI) has negligable effect, where there were the smallest differences in the obtained samples.
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Vliv chrómu na kvalitu ejakulátu a metabolismus sacharidů u plemenných kanců
Hánlová, Petra
Hánlová, P: Effect of chromium on semen quality and carbohydrate metabolism in bre-eding boars, Diploma Thesis MENDELU, Brno, 2015, s. 59 The aim of this thesis was to develop a literature search and experimentally de-termine the influence of mineral nutrition on boar semen quality. The thesis is divided into two parts. The first assesses the impact of the addition of chromium on semen qua-lity of breeding boars. The second part examines the influence of the addition of chro-mium on glucose and insulin levels in the blood of breeding boars. The experiment was conducted in Velké Meziříčí at the insemination center boars. The experiment consisted of 28 Duroc boars breed for 14 weeks. These boars were divided into 4 groups according to age balanced. In the control (n = 7) group (K), was chromium receiving 205 ug/kg/day. In the 1st group (n = 7) animals (CR1) was dose chromium even increased by 60.6 ug/kg compound feed for the second group (n = 7) boars (Cr2) of 121.2 ug/kg and 3. group (n = 7) boars (Cr3) was added chromi-um in an amount of 181.8 ug/kg compound feed. The addition of chromium to the semen quality of breeding boars too had a signifi-cant impact. During the first months of feeding chromium occurred in the control group to a decrease in ejaculate volume of 29 ml. In the group S3 during the experiment is bound to increase sperm concentration, but in the fourth taking under review decreased significantly compared to previous months. The difference between the third and fourth taking was 131.5 thousand/mm3. This difference is statistically insignificant, and could be caused by external influences or errors in nutrition. Sperm motility during certain months was below 70%. This ejaculate should not be used for insemination. In the second part of the experiment, the addition of the chromium significantly reduced glukose levels and insulin activity. The obtained results can not recommend feeding chromium breeding boars.
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Frekvence výskytu morfologických změn u aviárních spermií (gallus domesticus)
Freislebenová, Jana
Bachelor thesis describes the sexual organs of chickens, the composition of semen and its properties, by the way of sampling and examination methods used to analyze qualitative indicators of the semen. Furthermore, the work deals with the morphological structure of the sperm and the impacts of potential abnormalities in sperm morphology to fertility of the cocks. In the following thesis will be acquired and subsequently processed results and conclusions of a systematic investigation of individual semen samples using investigative methods, focusing on pathologies in sperm morphology and frequency of thein occurrence.
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Aspekty spermatologického vyšetření nativního bovinního ejakulátu
Weberová, Gabriela
In this bachelor thesis was described the reproductive system of a bull, hormonal regulation, sperm production and sperm, was also focused on the collection and sampling methods ejaculate. Work dealt with of studies of morphologically abnormal sperm and their influence on the fertility of bulls. At morphological defects been described primary and secondarily changed spermatozoa. Examination of native ejaculate was divided into microscopical, microbiological and special. At the end of this bachelor thesis was given the chapter on automated scoring systems ejaculates. Of those systems been described system for evaluating ejaculate CASA and flow cytometry.
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Hodnocení kvalitativních parametrů ejakulátu psů
Vágenknechtová, Marie
The dog has been accompanying men for eons, according to some literary sources the dog is the very first domesticated animal. My study included 152 dogs of 43 breeds. n total we had 408 ejaculates. We evaluated the proces of collection. Each ejaculate was evaluated on content, sperm count and motility of semen and morphological examination of semen.The evaluation of content and quality of ejaculate brought following average results. Time of ejaculation was 413,16 +- 168,66 seconds; the volume of ejaculate was 8,49 +- 6,51 ml; motility of semen 71,2 +- 16,8 %. There was approximately 69,1 +- 15,7 % of morphologically normal semen in the evaluated ejaculates. The results show, that you can expect quality ejaculate from middle sized sporting dogs. The dogs should be fed with a complete food mixture. The dog should be used for stud from the age of 2 years and in ideal conditions should not mate more often than once a week.
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