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Analysis of anti HLA antibodies using luminex (XMAP) methods in patients before and after kidney allotransplantation
Holoubková, Renata ; Jankovičová, Karolína (advisor) ; Andrýs, Ctirad (referee)
This work focuses on mastering the technique of examining anti-HLA antibodies using the multiplex Luminex method. The aim was to become proficient in working with analytical software capable of evaluating the specificity and levels of individual anti-HLA antibodies in patients' sera before and after kidney transplantation. Additionally, cut-off values for screening for anti-HLA antibodies in the assessed patient cohort were determined. Furthermore, an analysis of sensitization to individual HLA antigens was performed in a group of patients with low antibody levels. In the theoretical part of this work, I addressed the major histocompatibility system (HLA system) and its significance in transplantology. I also focused on the issues of transplant immunity and the principles of alloreactivity and rejection. I discussed the origin and significance of HLA antibodies and examined various methods for analyzing anti-HLA antibodies, particularly the multiplex Luminex method. In the experimental part, conducted at the Department of Clinical Immunology and Allergology in Hradec Kralove, I analyzed antibodies targeting HLA class I and II antigens using the multiplex Luminex method. Screening index values were monitored for both HLA classes. In subsequent typing examinations, MFI values were observed for...
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Humoral rejection after kidney transplantation and monitoring antibodies against HLA and non-HLA antigens.
Valhová, Šárka ; Slavčev, Antonij (advisor) ; Mrázek, František (referee)
Kidney transplantation is the treatment of choice for patients with end stage renal failure and is associated with prolonged survival of patients and better quality of life than long-term dialysis. Simultaneously, however, transplantation carries the risk of immunological complications leading to graft rejection. A serious problem in patients after organ transplantation is the development of humoral rejection, which is most often associated with the presence of antibodies specific to HLA antigens, particularly against mismatched HLA antigens of the organ donor. In certain cases antibodies may be specific to antigens expressed on endothelial cells, not on lymphocytes, like MICA, MICB, ICAM, and up till now unidentified tissue-specific antigens. Humoral rejection has significantly worse prognosis for the transplanted kidney than cellular rejection, and therefore its timely diagnosis is of great importance for the subsequent choice of appropriate therapy. The diagnosis of humoral rejection is based on the simultaneous detection of C4d deposits in the peritubular capillaries of the transplanted kidney and the finding of antibodies specific to the mismatched antigens of the donor (donor specific antibodies, DSA). The aim of our retrospective study was to contribute to improvement of the diagnosis of acute and...
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Humoral rejection after kidney transplantation and monitoring antibodies against HLA and non-HLA antigens.
Valhová, Šárka ; Slavčev, Antonij (advisor) ; Mrázek, František (referee)
Kidney transplantation is the treatment of choice for patients with end stage renal failure and is associated with prolonged survival of patients and better quality of life than long-term dialysis. Simultaneously, however, transplantation carries the risk of immunological complications leading to graft rejection. A serious problem in patients after organ transplantation is the development of humoral rejection, which is most often associated with the presence of antibodies specific to HLA antigens, particularly against mismatched HLA antigens of the organ donor. In certain cases antibodies may be specific to antigens expressed on endothelial cells, not on lymphocytes, like MICA, MICB, ICAM, and up till now unidentified tissue-specific antigens. Humoral rejection has significantly worse prognosis for the transplanted kidney than cellular rejection, and therefore its timely diagnosis is of great importance for the subsequent choice of appropriate therapy. The diagnosis of humoral rejection is based on the simultaneous detection of C4d deposits in the peritubular capillaries of the transplanted kidney and the finding of antibodies specific to the mismatched antigens of the donor (donor specific antibodies, DSA). The aim of our retrospective study was to contribute to improvement of the diagnosis of acute and...
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