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National Repository of Grey Literature

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Analysis of defensive substances of Diving beetles (Dytiscidae) by ultra-high performance liquid chromatography with mass spectrometry Bosáková, Tereza ; Kozlík, Petr (advisor) ; Nesměrák, Karel (referee) Obranný sekret potápníků (Dytiscidae) produkovaný jejich prothorakálními žlázami obsahuje řadu steroidních látek. Pro analýzu obranného sekretu potápníka druhu Ilybius fenestratus byla využita ultra- vysokoúčinná kapalinová chromatografie ve spojení s detekcí diodovým polem a hmotnostní detekcí. Analýza methanolického extraktu sekretu probíhala na koloně Acquity BEH C18 za podmínek gradientové eluce s dvousložkovou mobilní fází tvořenou methanolem s 0,1% obsahem amoniaku a 0,1% vodným roztokem amoniaku. V sekretu byla zjištěna přítomnost dvou steroidních hormonů, a to boldenonu a testosteronu a jejich identifikace byla potvrzena na základě shody jejich retenčních časů i hmotnostních spekter s příslušnými standardy. Detailed record

Color of Body by Dytiscidae: Variability and Possible Function HOKROVÁ, Monika The aim of this thesis was to establish a photographic database of selected species of diving beetles (Coleoptera: Dytiscidae) and use image analysis to characterize the number and extent of colour spots. Altogether 52 species were analyzed. They were classified into fourgroups based on their environmental characteristics provided in the Catalogue of water beetles of Czech Republic. The results confirmedthe hypothesis that the colour of adult diving beetles is correlated to their habitat preferences. Detailed record

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