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Methods for study of soil fungal communities - application on soils disturbed by outdoor cattle husbandry
Jirout, Jiří ; Elhottová, Dana
Fungal organisms comprise a physiologically and genetically diverse group of organisms with the key role in soil ecosystems. However, dynamics, structure, diversity and biomass of populations of soil fungi remain unclear and unknown. Conventional methods in microbiology provide only a glimpse view on the fungal diversity, but the last 15 years have brought many technological advances in community profiling and cultivation independent approaches to studying soil microorganisms. Changes in fungal diversity associated with agricultural management may affect the soil fertility and stability, but the effect of the agricultural practices on fungal complex community is still unclear. Conventional and modern methods in soil microbial ecology were used for study of microbial communities at the pasture used as a cattle overwintering area. Soil environment at this site is relevantly disturbed by cattle impact.

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