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Kvantitativní zastoupení bakterií rodu Lactobacillus v lidském zažívacím traktu v závislosti na změně stravovacích návyků
Kolísková, Kateřina
This diploma thesis deals with the issue of quantitative representation of the bacterial family in the human digestion system depending on changes in eating habits. The start of literary digest addresses general characteristics of digestion microbiome and its bacterial represen-tation. The most comprehensive part belongs to individual factors, who influenced overall number of bacterial species inhabiting the digestion microbiome. The last chapters of the theoretical part belong to cultivative and molecular biological methods, which are used in examination of the human digestion tract. Experimental part focuses on differences in the number of individual bacteria in the samples of human excrements, whose presence was determined by cultivative methods. Isolation of bacterial DNA was performed, which was subsequently confirmed by polymerase chain reaction. In resulting values, we observed small changes (p < 0,05) in bacterial species L. acidophilus a L. rhamnosus at the end of the second period. All bacterial families, except Bacteroides, had downward tendency (p > 0,05). Based on acquired data from food records, we observed changes in consumption of basic macronutrients (protein, fat, carbohydrates) and fiber. Only in carbohydrates and sim-ple sugars, we observed statistically provable differences (p > 0,05). Final analysis proved that changes in eating habits did not influence the total number of bacterial species inhabiting digestion microbiome.
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