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Utilization of light scattering techniques in study on protein denaturation
Köbölová, Klaudia ; Kalina, Michal (referee) ; Sedláček, Petr (advisor)
This bachelor thesis is focused on verifying the use of light scattering techniques in the study of protein denaturation. The theoretical part of the thesis describes proteins, their general properties, the process of denaturation and it provides an insight into light scattering. In the experimental part of the study I investigated the denaturation effects of four selected potential denaturing agents – urea, guanidine hydrochloride, sodium dodecyl sulfate, copper (II) sulfate – on two model enzymes: lipase and lysosyme. The denaturation effects of these denaturants were studied with the help of the dynamic light scattering method (DLS). In order to describe the change in particle size during denaturation, I used Z-Average, Volume mean and intensity distribution which were determined by this method.
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Drying of the organic materials
Fialová, Gabriela ; Lacina,, Lubor (referee) ; Svěrák, Tomáš (advisor)
This master’s thesis deals with the general characteristics of drying processes, types of moisture and bonds in the material. The following describes the drying process and selected drying equipment. It also includes a chapter on proteins, their changes in the air with increasing temperature. This thesis also describes denaturation of proteins as well as activity of water and it‘s dependence on microbial reactions in food. The practical part is focused on experimental measurement of the drying curves of crushed larvae, their construction and subsequent evaluation. The last part is devoted to measuring the activity of water samples of crushed larvae of different moisture.
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Proteins - New educational materials for education in biochemistry at secondary level
Fendrychová, Anna ; Martínek, Václav (advisor) ; Teplá, Milada (referee)
Diploma thesis is focused on creation of educational materials supporting the education of biochemistry, specifically amino acids and proteins, at secondary level. At first the analysis of Czech chemistry textbooks concerning the two topics - amino acids and proteins was performed. The major problems found were related to the insufficient graphical representation of biomolecules, unsatisfactory motivational components and insufficient integration of the topic with biology or everyday life experience. The supporting educational materials, presented in this work, supplement the widely used chemistry textbooks. The materials includes a graphic oriented presentation, interactive animations demonstrating the process of denaturation and precipitating of proteins at macroscopic and molecular level, poster presenting the structural formulas of standard amino acids, 3D models of selected proteins, additional texts supporting the current topics concerning amino acids and proteins and the laboratory protocols for students. The presented support materials were evaluated at the secondary school conditions. They were tested in one class and the improvement of student's understanding of the topic was compared to the second class employing only the classical educational methods. The comparison of the results of...
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Drying of the organic materials
Fialová, Gabriela ; Lacina,, Lubor (referee) ; Svěrák, Tomáš (advisor)
This master’s thesis deals with the general characteristics of drying processes, types of moisture and bonds in the material. The following describes the drying process and selected drying equipment. It also includes a chapter on proteins, their changes in the air with increasing temperature. This thesis also describes denaturation of proteins as well as activity of water and it‘s dependence on microbial reactions in food. The practical part is focused on experimental measurement of the drying curves of crushed larvae, their construction and subsequent evaluation. The last part is devoted to measuring the activity of water samples of crushed larvae of different moisture.
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Utilization of light scattering techniques in study on protein denaturation
Köbölová, Klaudia ; Kalina, Michal (referee) ; Sedláček, Petr (advisor)
This bachelor thesis is focused on verifying the use of light scattering techniques in the study of protein denaturation. The theoretical part of the thesis describes proteins, their general properties, the process of denaturation and it provides an insight into light scattering. In the experimental part of the study I investigated the denaturation effects of four selected potential denaturing agents – urea, guanidine hydrochloride, sodium dodecyl sulfate, copper (II) sulfate – on two model enzymes: lipase and lysosyme. The denaturation effects of these denaturants were studied with the help of the dynamic light scattering method (DLS). In order to describe the change in particle size during denaturation, I used Z-Average, Volume mean and intensity distribution which were determined by this method.
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Proteins - New educational materials for education in biochemistry at secondary level
Fendrychová, Anna ; Teplá, Milada (referee) ; Martínek, Václav (advisor)
Diploma thesis is focused on creation of educational materials supporting the education of biochemistry, specifically amino acids and proteins, at secondary level. At first the analysis of Czech chemistry textbooks concerning the two topics - amino acids and proteins was performed. The major problems found were related to the insufficient graphical representation of biomolecules, unsatisfactory motivational components and insufficient integration of the topic with biology or everyday life experience. The supporting educational materials, presented in this work, supplement the widely used chemistry textbooks. The materials includes a graphic oriented presentation, interactive animations demonstrating the process of denaturation and precipitating of proteins at macroscopic and molecular level, poster presenting the structural formulas of standard amino acids, 3D models of selected proteins, additional texts supporting the current topics concerning amino acids and proteins and the laboratory protocols for students. The presented support materials were evaluated at the secondary school conditions. They were tested in one class and the improvement of student's understanding of the topic was compared to the second class employing only the classical educational methods. The comparison of the results of...
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Design of technological set of device for production of bioethanol with a yearly capacity up to 500 000 litres.
SLANINKA, Milan
This bachelor thesis deals with the production of bioethanol for the use as fuel. The first theoretical part describes the process of the bioethanol production from the commonly grown cereals, then it focuses on the sources and characteristics of materials that can be used for the bioethanol production, it also follows the legal burden put on such production. The other part of the thesis contains the design of the technological set of devices for the bioethanol production with a given capacity of the yearly production.
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