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Study of the use of crops for phytoremediation of contaminated soil by pharmaceuticals
Hamplová, Marie ; Zlámalová Gargošová, Helena (referee) ; Mravcová, Ludmila (advisor)
Pharmaceuticals leaking into the environment from wastewater and livestock farms raise concerns about the impact on the ecosystem and human health. Residues of pharmaceuticals present in soil are subject to a number of biotic and abiotic processes, including uptake, translocation and accumulation by plants. The ability of plants to accumulate or degrade pharmaceuticals could have potential applications in the process of 'phytoremediation', where plants and their associated micro-organisms are used to stabilise, degrade or remove contaminants from the environment. In the framework of this thesis, experiments on phytoremediation of drug-contaminated soil by the cover crop oilseed radish (Raphanus sativus L. var. Oleiformis) under controlled conditions were conducted. Soil samples were extracted by ultrasonic extraction, plant samples by QuEChERS method and the final analysis of drugs was performed by UPLC-MS/MS. Oilseed radish produced a large amount of aboveground biomass compared to the other two crops and the presence of drugs had no negative effect on its growth. However, the determined levels of each drug in the soil and in the crop showed insufficient effectiveness of phytoremediation. The accumulation of drugs by the crop was low except for residues of the antidepressant venlafaxine. Therefore, according to these results, oilseed radish is not a suitable crop for phytoremediation of drug-contaminated soil. Experiments were also carried out with the root vegetable radish (Raphanus sativus L. var. Sativus) and carrot (Daucus carota L.) to assess the risk to human health associated with the accumulation of drugs in vegetable roots. The risk has not been demonstrated in terms of the amount of established drugs in the roots. Only the metabolite clofibric acid accumulated to a higher extent and the antibiotic trimethoprim was the most translocated to the aerial parts of the crop. The rate of uptake and translocation of drugs by the plant depended on many factors, as their content varied considerably between drug groups and between parts and types of crops.
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Vliv druhově bohatých meziplodin na půdu
Žák, Oldřich
Sufficient organic matter in the soil is necessary to maintain soil fertility. Numerous studies show that using cover crop mixtures can better prevent erosion, increase yields of the main crop, reduce pesticides and herbicides, reduce fertiliser, reduce diseases and pests, ultimately prevent nitrogen leaching and improve the overall impact of farming on biodiversity. Our experiment suggests that the impact of species-rich cover crops could be positive if proper management is followed.
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Půdoochranné technologie pěstování kukuřice s výsevem do mulče meziplodin
Reiter, Michal
The aim of this diploma thesis was to evaluate soil conservation technologies in the maize cultivation using cover crops. Biomass production was studied in selected cover crops. The influence of various tillage technologies and cover crops on the maize grain yield was monitored, and the number of maize plants in a row was evaluated. The exper-iment took place in 2018/2019 in Žabčice, at the school farm of Mendel University. Five types of cover crops were covered in the experiment: green peas, oats, mustard, phacelia and lucerne. Three options of tillage were studied: strip tillage, minimum tillage and no tillage. The oats showed the largest production of biomass. The largest production of maize grain was without using any cover crops, but some cover crops had almost the same grain production. Out of the cover crops, the best influence on the yield was by oats, otherwise the lowest influence on the yield was by lucerne. Maize achieved the highest yield after the minimum tillage technology.
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Interactive effects of adaptation technology, based on no-till sowing into the mulch of cover crop residues, and nitrogen nutrition on photosynthetic performance of maize under drought stress
Opoku, Emmanuel ; Holub, Petr ; Findurová, Hana ; Veselá, Barbora ; Klem, Karel
The aim of this study was to evaluate the interactive effect of adaptation technology based on no-till sowing into cover crop mulch and nitrogen nutrition on photosynthetic performance of maize under short term drought stress induced by rain-out shelters. The experiment was established in two locations in the same climatic condition but differing in soil fertility. The negative effect of drought on CO2 assimilation rate was modulated by nitrogen nutrition. However, while nitrogen nutrition led to alleviating effect at the location with higher fertility, the opposite effect was found at the site with lower fertility. Adaptation technology had only a minor impact on photosynthetic response to drought, but it generally increased CO2 assimilation rate at the site with higher soil fertility and decreased at the site with lower soil fertility. We can conclude that adaptation technology, despite of assumptions, did not significantly change the resilience of maize to drought, and probably longer use of such technology is required to improve soil water retention and thus also balanced supply of water to plants. \nAt the same time, we did not find a negative impact of adaptation technology on photosynthesis which can be related to cooler soil during maize emergence and slower mineralization, although the use of adaptation technology seems to be more effective in soils with higher fertility.
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The analysis of growing of legumes in selected farm and recommendations for it´s improvement
KASÍK, Jakub
The bachelor thesis deals with the cultivation of the main clover crops in the Czech Republic, which are alfalfa (Medicago sativa L.) and meadow clover (Trifolium pratense L.). In the first part of the thesis, the importance, characteristics, environmental requirements, agrotechnical measures and forage quality of these clovers are presented. The advantage of alfalfa is its hardiness and drought tolerance; it is still more damaged by waterlogging than by drought. In contrast, meadow clover has lower heat requirements and is better able to tolerate temporary waterlogging than a lack of moisture. Because of its slow initial growth, clover is often established in cover crops to compensate for forage yield in the year of establishment while suppressing weeds. The most suitable cover crop appears to be pea tendrils or a mixture with other crops, but in practice cereals are most commonly used. The second part focuses on the actual monitoring and evaluation of forage stands of meadow clover and alfalfa sown within the Zemědělské družstvo Čížová farming in the South Bohemian Region (potato-growing area). The method of establishment, number of plants per 1 m2 and yield of newly established stands of meadow clover were monitored in the operational plots. Meadow clover and alfalfa stands sown in the first crop year were also evaluated. Alfalfa had the highest dry matter yield (8.8 t ha-1), while clover had a slightly lower yield (8.2 t ha-1). The cover crop accounted for 60 % of the total dry matter yield of the established clover stands. It was found that at a stand density of 170 plants for alfalfa and 160 plants for clover, there should be no weeds in the stand.
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