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National Repository of Grey Literature

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Development of a fast method for site-directed mutagenesis in Streptococcus zooepidemicus Černý, Zbyněk ; Španová, Alena (referee) ; Pepeliaev,, Stanislav (advisor) This diploma thesis is focused on development of a fast method for site-directed gene mutagenesis in Streptococcus zooepidemicus based on the mechanism of natural competence. Several genes were selected based on experimental data which highly probably influence hyaluronic acid synthesis. The deletion of the selected genes from genomic DNA was performed as proof of concept, and the resulting recombinant strains were characterized regarding changes of hyaluronic acid precursor concentrations (glucuronic acid and N-acetylglucosamin) in time of cultivation and the end production of hyaluronic acid. Detailed record

Development of a fast method for site-directed mutagenesis in Streptococcus zooepidemicus Černý, Zbyněk ; Španová, Alena (referee) ; Pepeliaev,, Stanislav (advisor) This diploma thesis is focused on development of a fast method for site-directed gene mutagenesis in Streptococcus zooepidemicus based on the mechanism of natural competence. Several genes were selected based on experimental data which highly probably influence hyaluronic acid synthesis. The deletion of the selected genes from genomic DNA was performed as proof of concept, and the resulting recombinant strains were characterized regarding changes of hyaluronic acid precursor concentrations (glucuronic acid and N-acetylglucosamin) in time of cultivation and the end production of hyaluronic acid. Detailed record

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