National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Analýza vybraných W obohacených repetic u předivky brslenové \kur{Yponomeuta cagnagella} (Lepidoptera)
PILÍKOVÁ, Aneta
This thesis focuses on the repetitive sequences located in the W chromosome of the spindle ermine moth (Yponomeuta cagnagella) (Lepidoptera). The copy number of the selected repeats was established in male and female genomes from two geographic populations using real-time PCR (qPCR). Fluorescence in situ hybridization (FISH) was used to localize these sequences in female pachytene nuclei. The satellite tandem repeat and the transposon Maverick are present in the W chromosome in high copy numbers. In contrast to previously conducted bioinformatical analyses, the rDNA cluster is probably localized in an autosome in Y. cagnagella. The Maverick transposon was detected in both extensive terminal regions of the W chromosome.
New insights into the evolution of the W chromosome in Lepidoptera
HEJNÍČKOVÁ, Martina
It is generally accepted that the W chromosome in moths and butterflies (Lepidoptera) has evolved in a common ancestor of Tischeriidae and Ditrysia. Several species of these clades were examined via comparative genomic hybridization to determine the presence of the W chromosome. In addition, in five selected genes their Z-linkage was tested by qPCR. The conserved synteny of the Z chromosome across Tischeriidae and Ditrysia, along with the W chromosome absence in the lower ditrysian families Psychidae and Tineidae, suggests a possible independent origin of the W chromosomes in these two lineages.
Analýza karyotypu vakonošů (Psychidae, Lepidoptera) metodami klasické a molekulární cytogenetiky
HEJNÍČKOVÁ, Martina
Due to their phylogenetic position, Psychidae play an important role in the investigation of the W chromosome origin in Lepidoptera. Several species of Psychidae were tested for the presence of sex-chromatin and investigated via comparative genomic hybridization. Furthermore, odd chromosome numbers and a Z univalent were observed in females. Overall, this study brings tangible evidence for the absence of the W chromosome in Psychidae, thus contributes to complex knowledge of the W chromosome evolution. In addition, karyotypes of the given species were analyzed using 18S rDNA and histone H3 probes. The results indicate relative stability of their karyotypes.

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