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Measurement and Computer Simulation of Electromagnetic Oscillations of Living Cells
Cifra, Michal ; Pokorný, Jiří ; Vrba, J.
Frohlich postulated coherent polar oscillations as a fundamental biophysical property of biological systems. Recently, Pelling et al. (2004, 2005) detected mechanical vibrations of yeast cell membrane with atomic force microscope (AFM) and analyzed by Fourier analysis in the frequency range 0.5-2 kHz with amplitudes of the order of 1 nm. This article describes the measurement of electric activity of yeast cells in the acoustic frequency range and of mechanical vibrations of cell membrane. Spectrum analyzer and electrically and > electromagnetically screened box with point sensor and amplifiers fed by batteries were used for measurement of synchronized and non synchronized tubulin mutants of yeast cells. We show that the electric activity of synchronized cells in the M phase is greater that of non synchronized cells. That corresponds to the findings of Pohl et al. (1981). Obtained results of measurement of cell electric activity are in good agreement with AFM findings.
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Ultra low frequency yeast cells electric activity
Cifra, Michal ; Pokorný, Jiří ; Jelínek, František ; Hašek, Jiří ; Šimša, Jan
This paper presents results from the measurement of the electrical activity cold sensitive beta-tubulin mutant tub2-401 of yeast cells in frequency region 0.4 - 1.6 kHz. 25 measurements of both synchronized and nonsynchronized cells show that the synchronized cells have higher electrical activity what corresponds to the findings of Pohl et al. Mechanical oscillations of electrically polar structures in cell may give rise to electric oscillations we measured.
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Electromagnetic field around yeast cells
Pokorný, Jiří ; Jelínek, František ; Šaroch, Jaroslav ; Hašek, Jiří ; Palán, B.
Electromagnetic field around synchronized cells of tubulin mutant tub2-401 of Saccharomyces cerevisiae was measured in the M phase of the cell cycle in the frequency range of 8-9 MHz. The measured electromagnetic activity is compared with the evolution of the mitotic spindle. The peaks of the electromagnetic activity seems to coincide with formation of the mitotic spindle, with binding of chromatids to kinetochore microtubules, with anaphase A, and with anaphase B.
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Cavity modes of microtubules and biological cells in X-ray and infrared region
Jelínek, František ; Pokorný, Jiří
The microtubules in the cellular cytoskeleton have fundamental role in living processes of biological cells. They are hollow cylinders which resemble circular waveguides. The cutoff frequencies of the TM and TE modes of the microtubule cavities are in the band of the soft X-rays. This suggests the possibility of interaction of electromagnetic cavity modes with inner electrons in atoms. In the case of biological cells as cavity resonators the resonant frequencies may be in the infrared region.
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