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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Regulation of polyhydroxybutyrate production in Wautersia eutropha
 Grossová, Marie ; Čarnecká, Martina (referee) ; Márová, Ivana (advisor)
The aim of this work is study of microbial production of biodegradable polymers (polyhydroxyalkanoates; polyhydroxybutyrate PHB). Bacterium Wautersia eutropha was used for laboratory production of PHB. In this bacterium application of exogenous stress factors (osmotic shock, hydrogen peroxide and ethanol) was tested to enhance PHB production. Influence of exogenous stress on bacterial culture depended on stress factor concentration and time of stress application. Substantial influence on PHB yield exhibited changes of biomass production during stress experiments. The main part of this work is focused on study of PHB production in microbial cells exposed to stress in different growth phases. Application of strong stress in early phases of growth led to PHB and biomass decrease. The most suitable seems to be application of stress at the beginning of stationary phase accompanied by increase of PHB formation. Concentration of stress factor could be optimized too. The highest yields were obtained after addition of 0.5 % ethanol into production medium at 60th hour (PHB increase 21 %). These results can form a basis for new fermentation strategy which leads to enhanced effectiveness of PHB production.
Detailed record
Regulation of polyhydroxybutyrate production in Wautersia eutropha
 Grossová, Marie ; Čarnecká, Martina (referee) ; Márová, Ivana (advisor)
The aim of this work is study of microbial production of biodegradable polymers (polyhydroxyalkanoates; polyhydroxybutyrate PHB). Bacterium Wautersia eutropha was used for laboratory production of PHB. In this bacterium application of exogenous stress factors (osmotic shock, hydrogen peroxide and ethanol) was tested to enhance PHB production. Influence of exogenous stress on bacterial culture depended on stress factor concentration and time of stress application. Substantial influence on PHB yield exhibited changes of biomass production during stress experiments. The main part of this work is focused on study of PHB production in microbial cells exposed to stress in different growth phases. Application of strong stress in early phases of growth led to PHB and biomass decrease. The most suitable seems to be application of stress at the beginning of stationary phase accompanied by increase of PHB formation. Concentration of stress factor could be optimized too. The highest yields were obtained after addition of 0.5 % ethanol into production medium at 60th hour (PHB increase 21 %). These results can form a basis for new fermentation strategy which leads to enhanced effectiveness of PHB production.
Detailed record

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