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Recovery of waste from processing of phosphorus to produce materials with increased added value
Hejsek, Michal ; Zavřel, Tomáš (referee) ; Ing.Jan Červený, Ph.D. (advisor)
This master thesis focuses on reuse of waste water from industrial plant processing yellow phosphorus. Theoretical part summarizes physical and chemical properties of phosphorus, its transport in biosphere and its role in living organisms. Also the production process of the industrial plant is described. Waste water that contains waste byproducts is considered to be useful source of essential nutrients for economical large scale microalgae cultivation and development of biotechnological processes. In practical part, optimization of growth conditions for microalgae Chlorella pyrenoidosa Chick (IPPAS C-2) cultivation in medium based on wastewater from yellow phosphorus warehouse is presented.
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CO2 reduction with algae
Naider, Jakub ; Rebej, Miroslav (referee) ; Vondál, Jiří (advisor)
The aim of this final thesis is the process of algae cultivation their use for carbon dioxid reduction. The main objective of this work is the mass and heat balance of the photobioreactor and the design of a temperature control system. Within the research work, an overview of certain types of bioreactors and photobioreactors, the current situation around the climate and the impact of the cultivation of algae on carbon dioxide emissions. The model for temperature control and mass transfer of a plate photobioreactor is created in this work.
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Application of selected food industry wastes in the biotechnology of carotenogenic yeasts with a focus on optimizing yield coefficients.
Obračaj, Jan ; Němcová, Andrea (referee) ; Szotkowski, Martin (advisor)
This diploma thesis is focused on cultivation of selected carotenogenic yeasts on industrial waste substrates. These microorganisms are able to produce valuable metabolites with use in many industrial sectors, which primarily include pharmaceutical and food industries. Within this experiment, it was necessary to map the growth of these microorganisms on various alcohols and on other components of these waste substrates. Subsequently, the yield coefficients and amount of produced metabolites were compared, within the different strains and used source of nutrients. In theoretical part of the thesis are described used strains of yeasts, studied metabolites and their methods of extraction and analysis. The studied metabolites include carotenoid pigments, ubiquinone, ergosterol, microbial lipids and -glucans. The experimental part contains list of the equipment used, laboratory techniques, laboratory and analytical methods, chemicals, and list of the strains of microorganisms used. The result section then compares the amount of metabolites produced within the strain used and substrate used for cultivation, which took place both on a small scale and on a larger scale – fermentors. The results of this work can provide valuable information with application in industrial biotechnology of carotenogenic yeasts and reduce their economic demandingness and at the same time provide aid with ecological disposal of selected industrial wastes.
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CO2 reduction with algae
Naider, Jakub ; Rebej, Miroslav (referee) ; Vondál, Jiří (advisor)
The aim of this final thesis is the process of algae cultivation their use for carbon dioxid reduction. The main objective of this work is the mass and heat balance of the photobioreactor and the design of a temperature control system. Within the research work, an overview of certain types of bioreactors and photobioreactors, the current situation around the climate and the impact of the cultivation of algae on carbon dioxide emissions. The model for temperature control and mass transfer of a plate photobioreactor is created in this work.
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Adhesion of microalgae Chlorella vulgaris to solid surfaces
Širmerová, Marcela ; Vymazal, Jan (advisor) ; Mojmír, Mojmír (referee)
Although adhesion of bacteria and yeast have been extensively studied by a wide range of experimental and theoretical approaches, significantly less attention has been focused on microalgal adhesion to solid materials. Therefore this doctoral thesis is focused on physico-chemical aspects of microalgal adhesion. The results are based on experimental characterization of surface properties of both microalgae and solids by contact angle and zeta potential measurements. These data were subsequently used in modeling the surface interactions (thermodynamic, classical and extended DLVO models) resulting in quantitative prediction of the interaction intensities. Finally, the model predictions are compared with experimental adhesion tests of microalgae onto model solids in order to identify the physico-chemical forces governing the microalgae-solid interaction. The model solids were prepared in order to cover a wide range of properties (hydrophobicity, surface charge). The results revealed that in low ionic strength environment the adhesion was influenced mostly by electrostatic attraction/repulsion between surfaces, while with increasing ionic strength grew the importance of apolar (hydrophobic) interactions. Among tested materials the highest adhesion of Chlorella vulgaris cells was observed to (3-aminopropyl)triethoxysilane modified glass (APTES glass) mimicking the surface properties of stainless steel. The impact of solid surface properties on the degree of colonization by microlagae was statistically more significant than the influence of culture medium composition on cell surface of Chlorella vulgaris.
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Recovery of waste from processing of phosphorus to produce materials with increased added value
Hejsek, Michal ; Zavřel, Tomáš (referee) ; Ing.Jan Červený, Ph.D. (advisor)
This master thesis focuses on reuse of waste water from industrial plant processing yellow phosphorus. Theoretical part summarizes physical and chemical properties of phosphorus, its transport in biosphere and its role in living organisms. Also the production process of the industrial plant is described. Waste water that contains waste byproducts is considered to be useful source of essential nutrients for economical large scale microalgae cultivation and development of biotechnological processes. In practical part, optimization of growth conditions for microalgae Chlorella pyrenoidosa Chick (IPPAS C-2) cultivation in medium based on wastewater from yellow phosphorus warehouse is presented.
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